PENN BIOENGINEERING:
Shared Equipment

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1470 Automatic Gamma Counter by Perkin Elmer
Location: 310 SKH

Description (from brochure):
The 1470 Automatic Gamma Counter can take multiple samples for radiometric detection.  The Gamma Counter uses a unique well-type detector and sample changer system along with highly effective lead shielding, resulting in high counting efficiency, constant background and minimal crosstalk.  This device has one detector.

Website: http://www.perkinelmer.com
Note that this device can no longer be found on the manufacturer’s website.

If you would like to use this equipment, your lab must have a radioactive materials handling license.

 

7300 Real-time PCR system by Applied Biosystems
Locations: 410 & 430 SKH

Description (from website):
This PCR has advanced multicolor detection capabilities for gene expression analysis, absolute quantitation, SNP genotyping and +/- assays utilizing internal positive controls.  This PCR uses a 96-well block and takes 0.2mL tubes.

Website: http://www.appliedbiosystems.com

 

Allegra 25R DNA Centrifuge by Beckman Coulter
Location: 540 SKH

Description (from website):
Operates at temperature from 2 to 40 C. It can reach speeds of 15K rpm.

Website: http://www.beckmancoulter.com

 

Avanti J-E Centrifuge by Beckman Coulter
Location: 430 SKH

Description (from website):
The Avanti J-E centrifuge operates at speeds of up to 21,000 rpm (53,300 x g).

Website: http://www.beckmancoulter.com

Rotors available:

JA-25.50 - Fixed Angle, Single Lock lid, 8x50ml, 25,000 rpm, 75,600xg (part# 363055)

JLA-16.250 - Fixed Angle, 16,000 rpm, 38,500xg (part# 363934)

 

Breeze HPLC for Gel Permeation Chromatography by Waters Corporation
Location: 435SKH

Description (from website):
The Breeze HPLC GPC System includes software, pump, detector and injector.  This Breeze system is configured for Gel Permeation Chromatography. 

Website: http://www.waters.com

 

CentriVap Speed Vacuum by Labconco
Location: 335 SKH

Description (from brochure):
CentriVap Centrifugal Vacuum Concentrators use a combination of centrifugal force, vacuum and heat for speed evaporation of multiple small samples. The CentriVap’s larger chamber and selection of higher capacity rotors offer the maximum throughput required by molecular biology, microbiology, biochemistry, pharmaceutical research and analytical chemistry laboratories. As many as 132 samples may be processed at once. Sample sizes range from a few microliters up to 25 milliliters. In addition, the Microtiter Plate Rotor holds four standard or two deep well plates.
The CentriVap also has a Cold Trap, protecting the accessory vacuum pump from the corrosive effects of vapors and fumes as they evaporate from the samples.

Website: http://www.labconco.com

 

Contact Angle Meter by TanTec, ChemInstruments
Location 430 SKH

Description (from website):
The ChemInstruments CAM-Plus contact angle meter combines reliable surface energy measurement with ease of use. The patented “Half Angle” measurement method eliminates the difficulties associated with tangent angle measurement methods.  With simple, reliable and consistent operation, one can quickly determine surface energy measurements to help in coating operations of all types.

Website: http://www.chemsultants.com/

 

Gene Pulser Xcell Electroporation System by Bio-Rad
Location: 510 SKH

Description (from website):
The Gene Pulser Xcell is a modular electroporation system, which includes a main unit, two accessory modules (the CE module and the PC module), and the ShockPod cuvette chamber. The CE module is recommended for use with the Gene Pulser Xcell main unit for electroporation of most eukaryotic cells, including mammalian cells and plant protoplasts. The PC module is recommended for electroporation of bacteria and fungi, as well as other applications where high-voltage pulses are applied to samples of small volume and high resistance.

Website: http://www.bio-rad.com

 

GloMax 20/20 Single Tube Luminometer by Promega
Location: Please contact Sevile Mannickarottu to request (sevile@seas.upenn.edu)

Description (from website):
The 20/20 Luminometer is designed to run bioluminescent assays, such as reporter gene assays, as well as chemiluminiscent assays.  A touchscreen interface provides comprehensive instrument control and data collection.

Website: http://www.promega.com

 

HPLC Series 1200 by Agilent
Location: 410 SKH

Description (from website):
The Agilent Series HPLC 1200 provides spectral information from 190 to 950nm, mass selective detection, reliable high sensitivity nanospray, superior injection precision with low carryover and high resolution chromatography.

Website: http://www.agilent.com

 

Innova 42R Incubator by Eppendorf
Location: 510 SKH

Description (from website):
The Innova incubator is a programmable shaker incubator.

Website: http://www.eppendorf.com

 

NanoDrop ND-1000 by NanoDrop
Location: 540 SKH

Description (from website):
The NanoDrop ND-1000 Spectrophotometer measures 1 µL samples with high accuracy and reproducibility. The full spectrum (220nm-750nm) spectrophotometer utilizes a patented sample retention technology that employs surface tension alone to hold the sample in place.

Website: http://www.nanodrop.com/

 

Nicolet 6700 FT-IR Spectrometer by Thermo Fisher Scientific
Location: 430 SKH

Description (from website):
The Nicolet 6700 FT-IR spectrometer can be configured for multiple spectral ranges (far-IR to UV-Vis), with the option of gold-coated or aluminum optics. The temperature of the source during normal operation is electronically controlled.  Users have additional options of 'Rest' and 'Turbo' mode. The former helps to extend the life of the source, while the latter provides extra output for difficult samples.

Website: http://www.thermo.com

 

Odyssey Infrared Imaging System by Li-Cor Biosciences
Location: 310 SKH

Description (from website):
The Odyssey Infrared Imaging System uses direct infrared fluorescence detection for Western blot analysis.  Infrared detection gives you the quantitative analysis and wide linear dynamic range that chemiluminescence can’t. Strong and weak bands on the same blot are accurately detected without the uncertainty and inconvenience of multiple exposures, and without spending time in the darkroom. The Odyssey System gives you clear, sharp, reproducible bands without fuzziness or “blowout”. Bands hidden by overexposure with chemiluminescence become clear when imaged with Odyssey.

Website: http://www.licor.com

 

Optima L-100 K BioSafe Centrifuge System by Beckman Coulter
Location: 430 SKH

Descriptions (from website):
The Optima L-100 K BioSafe Centrifuge is capable of generating 802,000 x g at speeds up to 100,000 rpm.

Website: http://www.beckmancoulter.com

Rotors available:

*SW 41 - Swinging Bucket, 6x13.2 mL, 41,000 rpm, 288,000xg (part# 331362) * Currently unavailable (8/11/2014)

SW 60 Ti - Swinging Bucket, Titanium 6x4ml, 60,000rpm, 485Kxg (part#335649)

 

P-1000 Flaming-Brown Micropipette Puller by Sutter Instruments
Location: 430 SKH

Description (from website):
The P-1000 Micropipette Puller fabricates micropipettes and offers a color touchscreen interface and an extensive library of programs found in the popular Sutter Cookbook. You need only specify the glass, filament, and type of pipette you require and a suitable program will be identified. Copy and paste functions assure that programs can be easily written and the line repeat mode simplifies multi-line programming. User notes can be  added to each program for annotating important information. Help topics are pre-loaded to assist with on-site troubleshooting and the built-in glossary includes text, pictures, and diagrams explaining the terms used in micropipette fabrication. A rotary dial is offered as an alternative to the keypad for numerical entry.

Website: http://www.sutter.com/

 

PS3 Peptide Synthesizer by Protein Technologies
Location: 430 SKH

Description (from website):
The PS3 is a simple peptide Synthesizer.  The PS3 has 3 serial reaction vessels and allows up to 45 couplings without user intervention or reloading.

Website: http://www.peptideinstruments.com/

 

Radiance 2000 Confocal Multiphoton System and Inverted Microscope by Bio-Rad
Location: 390 Towne

Description (from brochure):
The Radiance 2000 System by Bio-Rad provides a wide range of scan speeds (25-750Hz), three internal confocal detectors and full 360 degree scan rotation. The microscope which uses this system is a Nikon TE-300 Inverted Microscope.

Website: http://www.bio-rad.com

 

Zetasizer Nano Series by Malvern Instruments
Location: 335 SKH

Description (from website):
The Zetasizer Nano series can perform a combination of three parameters: particle size analysis, zeta potential measurement, and molecular weight measurement.  The analyzer can enable measurements of particles and molecules from 0.6nm to 6 microns.  The device provides accurate measurements of zeta potential in aqueous and non-aqueous dispersions.  An avalanche-photodiode detector and fiber detection optics give the sensitivity and stability required for absolute molecular weight measurements.

Website: http://www.malvern.com

 

 

 
 

Department of Bioengineering
School of Engineering and Applied Science
University of Pennsylvania
240 Skirkanich Hall
210 S. 33rd Street
Philadelphia, PA 19104

Last Update: July 10, 2009
Page Maintained by Sevile G. Mannickarottu