Andrew Tsourkas, Ph.D.
atsourk@seas.upenn.edu
Cellular
& Molecular Imaging Group Lab Page
Assistant Professor
of Bioengineering
Assistant Professor
of Radiology
Member, Institute
of Medicine and Engineering
B.S., Mechanical Engineering,
Cornell University
M.S., Mechanical Engineering, Johns Hopkins University
Ph.D., Biomedical Engineering, Georgia Tech/Emory University
Research Interests
The rapid advancement in our understanding
of the regulatory and signaling pathways responsible for cell growth,
differentiation, and death has led to the identification of an array
of anomalies in the genome and proteome that can be associated with
disease. Our laboratory is interested in developing molecular imaging
probes that target these anomalies to locate and study diseased
states in vivo. We are developing probes that target a wide range
of biological processes including gene regulation, mRNA localization,
protein expression, and enzymatic activity. Multiple imaging platforms
are used including magnetic resonance, fluorescence, and bioluminescence
and applications range from studying the complex intracellular dynamics
of individual cells to the early detection of disease in a clinical
setting.
Selected Publications
Tsourkas*, A., Shinde-Patil*,
V., Kelly, K., Patel, P., Wolley, A., Allport, J., Weissleder, R.
(2005) In vivo imaging of activated endothelium using an anti-VCAM-1
magneto-optical probe. /Bioconjugate Chemistry/, 16(3), 576-581.
Tsourkas, A., Newton, G., Perez, J., Basilion, J., Weissleder, R.
(2005) Detection of peroxidase/H2O2-mediated oxidation with enhanced
yellow fluorescent protein. /Analytical Chemistry/, 77(9),
2862-2867.
Kelly*, K., Allport,
J., Tsourkas, A., Shinde-Patil, V., Josephson, L., Weissleder, R.
(2005) VCAM-1-mediated cell internalization allows in vivo imaging
of activated endothelium. /Circulation Research/, 96, 327-336 (*contributed
equally).
Tsourkas, A., Hofstetter, O., Hofstetter,
H., Weissleder, R., Josephson, L. (2004) Magnetic relaxation switch
immunosensors detect enantiomeric impurities. /Angewandte Chemie
International Edition/, 43(18), 2395-2399.
Tsourkas, A., Weissleder,
R. (2004) Illuminating the dynamics of intracellular activity with
active' molecular reporters. /Mechanics and Chemistry of Biosystems/,
1(2), 133-145.
Perez, J., Simeone, F.,
Tsourkas, A., Josephson, L., Weissleder, R. (2004) Peroxidase substrate
nanosensors for MR imaging. /Nano Letters/, 4(1), 119-122.
Bao, G., Tsourkas, A.,
Santangelo, P. (2004) Engineering nanostructured probes for sensitive
intracellular gene detection. /Mechanics and Chemistry of Biosystems/,
1(1), 23-36.
Santangelo, P., Nix, B.,
Tsourkas, A., Bao, G. (2004) Dual FRET molecular beacons for mRNA
detection in living cells. /Nucleic Acids Research/, 32(6), e57.
Tsourkas, A., Behlke,
M., Xu, Y., Bao, G. (2003) Spectroscopic features of dual fluorescence/luminescence
resonance energy-transfer molecular beacons. /Analytical Chemistry/,
75(15), 3697-3703.
Tsourkas, A., Behlke,
M., Rose, S., Bao, G. (2003) Hybridization kinetics and thermodynamics
of* *molecular beacons. /Nucleic Acids Research/, 31(4), 1319-1330.
Tsourkas, A., Bao, G.
(2002) Shedding light on health and disease using molecular beacons.
/Briefings in Functional Genomics and Proteomics/, 1(4), 1-13.
Tsourkas, A., Behlke,
M., Bao, G. (2002) Structure-function relationships of shared-stem
and conventional molecular beacons. /Nucleic Acids Research/, 30(19),
4208-4215.
Tsourkas, A., Behlke,
M., Bao, G. (2002) Hybridization of 2'-O-methyl and 2'-deoxy molecular
beacons to RNA and DNA targets. /Nucleic Acids Research/, 30(23),
5168-5174.
Bioengineering
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