University of Pennsylvania · Department of Bioengineering

Publications

35) Elias, D.R., Cheng, Z., Tsourkas, A. (2010) An Intein-Mediated Site-Specific Click Bioconjugation Strategy for Improved Tumor Targeting of Nanoparticle Systems.  Small.  In press.

34) Chen, A., Davydenko, O., Behlke, M.A., Tsourkas, A. (2010) Ratiometric bimolecular beacons for the sensitive detection of RNA in single living cells.  Nucleic Acids Research, 38(14), e148. PMCID: PMC2919734

33) Cheng, Z., Chen, A., Lee, H.-Y., Tsourkas, A. (2010) Examination of folate-targetd liposomes with encapsulated poly(2-propylacrylic acid) as a pH-responsive platform for cytosolic drug delivery.  Small, 6(13), 1398-401.

32) Thorek, D.L.J., Tsao, P.Y., Arora, V., Zhou, L., Eisenberg, R.A., Tsourkas, A. (2010) In vivo multimodal imaging of B cell distribution and response to antibody immunotherapy in mice.  PLoS One, 5(5), e10655. PMCID: PMC2871797

31) Thorek, D.L.J, Weisshaar C.L., Czupryna, J.C., Winkelstein B.A., Tsourkas A. (2010) SPIO-enhanced detection of transient cervical nerve root compression.  Molecular Imaging, in press.

30) Cheng, Z., Thorek, D.L.J., Tsourkas, A. (2010) Gadolinium-conjugated dendrimer nanoclusters as a tumor targeted T1 magnetic resonance imaging contrast agent.  Angewandte Chemie International Edition, 49(2), 346-350.  PMCID: PMC2862691

29) Warden, R., Saunders, J., Tsourkas, A. (2009) Commentary: Two-photon imaging of multicellular tumor spheroids: a novel method for evaluating the efficacy of CDC25 inhibitors.  Cancer Biology and Therapy,  8(23), 2237-2239. 

28) Chen, A.K., Behlke, M.A., Tsourkas, A. (2009) Sub-cellular trafficking and functionality of 2’-O-methyl and 2’-O-methyl-phosphorothioate molecular beacons.  Nucleic Acids Research, 37(22), e149.  PMCID: PMC2794186

27) Chen, A.K., Tsourkas, A. (2009) Imaging RNA in living cells with molecular beacons: current perspective and challenges. Journal of Innovative Optical Health Sciences, 2(4), 315-324.

26) Cheng, Z., Thorek, D.L.J, Tsourkas, A. (2009) Porous polymersomes with encapsulated Gd-labeled dendrimers as highly efficient MRI contrast agents. Advanced Functional Materials, 19(23), 3753-3759.

25) Thorek, D.L.J., Elias, D.R, Tsourkas, A. (2009) Comparative analysis of nanoparticle-antibody conjugations: carbodiimide versus click chemistry. Molecular Imaging, 8(4), 221-229. 

24) Lu, J., Tsourkas, A. (2009) Imaging individual microRNAs in single mammalian cells in situ.  Nucleic Acids Research, 37(14), e100.  PMCID: PMC2724290

23) Bao, G., Rhee, W., Tsourkas, A. (2009) Fluorescent probes for live cell RNA detection.  Annual Reviews of Biomedical Engineering, 11, 25-47.  PMCID: PMC2734976

22) Chen, A.K., Cheng, Z., Behlke, M.A., Tsourkas, A. (2008) Assessing the sensitivity of commercially available fluorophores to the intracellular environment.  Analytical Chemistry, 80(19), 7437-7444.  PMCID: PMC2626168

21) Elias, D.R., Thorek, D.L.J., Chen, A.K., Czupryna, J., Tsourkas, A. (2008) In vivo imaging of cancer biomarkers using activatable molecular probes.  Cancer Biomarkers, 4(6), 287-305. 

20) Thorek, D.L.J., Tsourkas, A. (2008) Size, charge, and concentration dependent uptake of iron oxide nanoparticles by non-phagocytic cells: a comparative study of USPIO, SSPIO, and MPIO.  Biomaterials, 29(26), 3583-3590.  PMCID: PMC2518173

19) Chen, A.K., Behlke, M.A., Tsourkas, A. (2008) Efficient cytosolic delivery of molecular beacon conjugates and flow cytometric analysis of target RNA.  Nucleic Acids Research, 36(12), e69.  PMCID: PMC2475621

18) Cheng, Z., Tsourkas, A. (2008) Paramagnetic porous polymersomes. Langmuir, 24(15), 8169-8173.  PMCID: PMC2646750

17) Zhang, C.Y., Lu, J., Tsourkas, A. (2008) Iron chelator-based amplification strategy for improved targeting of transferrin receptor with SPIO.  Cancer Biology and Therapy, 7(6), 889-895. 

16) Chen, A.K., Behlke, M.A., Tsourkas, A. (2007) Avoiding false-positive signals with nuclease-vulnerable molecular beacons in single living cells.  Nucleic Acids Research, 35(16), e105.  PMCID: PMC2018645

15) Czupryna, J., Tsourkas, A. (2006) Commentary: Suicide gene delivery by calcium phosphate nanoparticles: a novel method of targeted therapy for gastric cancer.  Cancer Biology and Therapy, 5(12), 1691-1692. 

14) Thorek, D.L.J., Chen, A.K., Czupryna, J., Tsourkas, A. (2006) Superparamagnetic iron oxide nanoparticle probes for molecular imaging.  Annals of Biomedical Engineering, 34(1), 23-38. 

13) Tsourkas*, A., Shinde-Patil*, V., Wolley, A., Allport, J., Weissleder, R. (2005) In vivo imaging of activated endothelium using anti-VCAM-1 magnetooptical probes. Bioconjugate Chemistry, 16(3), 576-581 (*contributed equally). 

12) Tsourkas, A., Newton, G., Perez, J., Basilion, J., Weissleder, R. (2005) Detection of peroxidase/H2O2-mediated oxidation with enhanced yellow fluorescent protein.  Analytical Chemistry, 77(9), 2862-2867. 

11) Kelly*, K., Allport*, J., Tsourkas, A., Shinde-Patil, V., Josephson, L., Weissleder, R. (2005) Detection of vascular adhesion molecule-1 expression using a novel multimodal nanoparticle.  Circulation Research, 96, 327-336(*contributed equally). 

10) Tsourkas, A., Weissleder, R. (2004) Illuminating the dynamics of intracellular activity with ‘active’ molecular reporters.  Mechanics and Chemistry of Biosystems, 1(2), 133-145. 

9) Tsourkas, A., Hofstetter, O., Hofstetter, H., Weissleder, R., Josephson, L. (2004) Magnetic relaxation switch immunosensors detect enantiomeric impurities.  Angewandte Chemie International Edition, 43(18), 2395-2399. 

8) Perez, J., Simeone, F., Tsourkas, A., Josephson, L., Weissleder, R. (2004) Peroxidase substrate nanosensors for MR imaging.  Nano Letters, 4(1), 119-122.

7) Bao, G., Tsourkas, A., Santangelo, P. (2004) Engineering nanostructured probes for sensitive intracellular gene detection.  Mechanics and Chemistry of Biosystems, 1(1), 23-36. 

6) Santangelo, P., Nix, B., Tsourkas, A., Bao, G. (2004) Dual FRET molecular beacons for mRNA detection in living cells.  Nucleic Acids Research, 32(6), e57.  PMCID: PMC390379

5) Tsourkas, A., Behlke, M., Xu, Y., Bao, G. (2003) Spectroscopic features of dual fluorescence/luminescence resonance energy-transfer molecular beacons.  Analytical Chemistry, 75(15), 3697-3703. 

4) Tsourkas, A., Behlke, M., Rose, S., Bao, G. (2003) Hybridization kinetics and thermodynamics of molecular beacons.  Nucleic Acids Research, 31(4), 1319-1330.  PMCID: PMC150230

3) Tsourkas, A., Bao, G. (2002) Shedding light on health and disease using molecular beacons.  Briefings in Functional Genomics and Proteomics, 1(4), 1-13. 

2) Tsourkas, A., Behlke, M., Bao, G. (2002) Structure-function relationships of shared-stem and conventional molecular beacons.  Nucleic Acids Research, 30(19), 4208-4215.  PMCID: PMC140536

1) Tsourkas, A., Behlke, M., Bao, G. (2002) Hybridization of 2’-O-methyl and 2’-deoxy molecular beacons to RNA and DNA targets.  Nucleic Acids Research, 30(23), 5168-5174.  PMCID: PMC137950


Book Chapters

6) Chen, A.K., Rhee, W.J., Bao, G., Tsourkas, A. (2010) Delivery of molecular beacons for live-cell imaging and analysis of RNA In Methods in Molecular Biology: Messages on the move: Techniques in RNA visualization, edited by Gerst, J.E., The Humana Press Inc.

5) Crayton, S.H., Chen, A.K., Cheng, Z., Tsourkas, A. (2010) Molecular Imaging In Comprehensive Biomaterials, edited by Ducheyne, P., Healy, K., Hutmacher, D., Kirkpatrick, J., Elsevier Inc.  In press.

4) Lu, J., Tsourkas, A. (2009) Quantification of miRNA abundance in single cells using locked nucleic acid-FISH and enzyme labeled fluorescence In Methods in Molecular Biology: Molecular Imaging, edited by Marton, P., The Humana Press Inc.

3) Elias, A., Tsourkas, A. (2009) Imaging circulating cells and lymphoid tissues with iron oxide nanoparticles In Hematology American Society of Hematology Education Program, 720-726.

2) Tsourkas, A., Josephson, L. (2008) Magnetic Nanoparticles In Molecular Imaging: Principles and Practice, edited by Weissleder, R., Gambhir, S.S., Ross, B.D., Rehemtulla, A.  BC Decker Inc, Publisher.

1) Thorek, D.L.J., Czupryna, J., Chen, A.K., Tsourkas, A. (2006) Molecular imaging of cancer with superparamagnetic iron oxide nanoparticles In Cancer Imaging, edited by M.A. Hayat.  Elsevier Science/Academic Press.