University of Pennsylvania · Department of Bioengineering

Publications

79) Gao, Q., Yan, L., Chiorazzo, M., Delikatny, E.J., Tsourkas, A., Cheng, Z. (2015) PLA2-responsive and SPIO-loaded phospholipid micelles. Submitted.

78) Yan, L., Crayton, S.H., Thawani, J., Amirshaghaghi, A., Tsourkas, A., Cheng, Z. (2015) A pH-responsive drug delivery platform based on glycol chitosan-coated liposomes. Small, in press.

77) Warden-Rothman, R., Song, Y., Hui, J.Z., Tsourkas, A. (2015) Efficient production of modular bispecific targeting ligands with sortase-tag expressed protein ligation. Submitted.

76) Hui, J.Z., Tamsen, S., Song, Y., Tsourkas, A. (2015) LASIC: Light activated site-specific conjugation of native IgGs. Submitted.

75) Yan, L., Higbee, E., Liu, R., Tsourkas, A., Cheng, Z. (2015). A facile method for the synthesis of porous polymeric vesicles. Submitted.

74) Karunamuni, R., Al Zaki, A., Popov, A., Delikatny, J., Cormode, D., Tsourkas, A., Maidment, A.D.A. (2015) Development of silica-encapsulated silver nanoparticle as contrast agents in dual-energy mammography. Submitted.

73) Nwe, K., Huang, C-H., Qu, F., Warden-Rothman, R., Mauck, R.L., Tsourkas, A. (2015) Cationic gadolinium chelate for magnetic resonance imaging of cartilage defects. Submitted.

72) Giraldo-Vela, J.P., Kang, W., McNaughton, R., Zhang, X., Tsourkas, A., Bao, G., Espinosa, H.D. (2015) Single cell detection of mRNA expression using nanofountain-probe electroporated molecular beacons. Small, 11(20), 2386-2391.

71) Al Zaki, A., Hui, J.Z., Higbee, E., Tsourkas, A. (2015) Biodistribution and clearance of gold-loaded polymeric micelles using 0.9 and 5 nm gold nanoparticles. Journal of Biomedical Nanotechnology, In press.

70) Sun L., Joh D., Stangl M., Al Zaki A., Murty S., Alonso-Basanta M., Kao G, Tsourkas A., Dorsey J.F. (2015) Theranostic application of mixed gold and iron oxide nanoparticle micelles in glioblastoma multiforme. Journal of Biomedical Nanotechnology, in press.

69) Vargo, K.B., Al Zaki, A., Warden-Rothman, R., Tsourkas, A., Hammer, D.A. (2015) Superparamagnetic iron oxide nanoparticle-loaded micelles stabilized by recombinant oleosin for targeted magnetic resonance imaging. Small, 11(12), 1409-1413.

68) McQuade*, C., Al Zaki*, A., Desai, Y., Vido, M., Sakhuja, T., Cheng, Z., Hickey, R., Joh, D., Park, S-J, Kao, G.D., Dorsey, J.F., Tsourkas, A. (2015).  A multi-functional nanoplatform for imaging, radiotherapy, and the prediction of therapeutic response. Small, 11(7), 834-843 (*contributed equally). PMCID: PMC4329028.

67) Lajud, S.A., Nagda, D.A., Qiao, P., Tanaka, N., Civantos, A., Gu, R., Cheng, Z., Tsourkas, A., O’Malley, B.W., Li, D. (2015) A novel chitosan-hydrogel-based nanoparticle delivery system for local inner ear application. Otology & Neurology, 36(2), 341-341. PMCID:PMC4365436.

66) Murty, S., Gilliland, T., Qiao, P., Tabtieng, T., Higbee, E., Al Zaki, A., Puré, E., Tsourkas, A. (2014) Nanoparticles functionalized with collagenase exhibit improved tumor accumulation in a murine xenograft model. Particle and Particle Systems Characterization, 31, 1307-1312.

65) Eckmann, D.M., Composto, R.J., Tsourkas, A., Muzykantov, V.R. (2014) Nanogel carrier design for targeted drug delivery. Journal of Materials Chemistry B. 2, 8085-8097. PMCID: PMC4251498.

64) Hui, J.Z., Tsourkas, A. (2014) Optimization of photo-active Protein Z for fast and efficient site-specific conjugation of native IgG. Bioconjugate Chemistry, 25(9), 1709-1719. PMCID:PMC4166039.

63) Cheng, Z., Tsourkas, A. (2014) Monitoring phospholipase A2 activity with Gd-encapsulated phospholipid liposomes. Scientific Reports, 4, 6958. PMCID:PMC4223643.

62) Naha, P.C., Al Zaki, A., Hecht, E., Chorny, M., Chhour, P., Blankemeyer, E., Witschey, Wl, Litt, H.I., Tsourkas, A., Cormode, D.P. (2014) Dextran coated bismuth-iron oxide nanohybrid contrast agents for computed tomography and magnetic resonance imaging. Journal of Materials Chemistry B. 2, 8239-8248. PMCID: PMC4251562.

61) Chhour, P., Gallo, Cheheltani, R., Williams, D., Al Zaki, A., Paik, T, Nichol, J.L., Tian, Z., Naha, P.C., Allcock, H.R., Murray, C.B., Tsourkas, A., Cormode, D.P. (2014) Nano-disco balls: polyphosphazene nanospheres surface-loaded with diagnostically active nanocrystals. ACS Nano, 8(9), 9143-9153. PMCID:PMC4174093.

60) Karunamuni, R., Tsourkas, A., Maidment, A.D.A. (2014) Exploring silver as a contrast agent for contrast-enhanced dual-energy x-ray breast imaging. The British Journal of Radiology. 87(1041), 20140081.

59) Elias*, D.R., Crayton*, S.H., Warden, R., Wang, A. Tsourkas, A. (2014) Quantitative comparison of tumor delivery for multiple targeted nanoparticles simultaneously by Multiplex ICP-MS.  Scientific Reports, 4, 5840 (*contributed equally).

58) Hui, J.Z., Al Zaki, A., Cheng, Z., McNitt, C.D., Popik, V., Zhang, H., Luning Prak, E.T., Tsourkas, A. (2014) Facile method for the site-specific, covalent attachment of full-length IgG onto nanoparticles. Small, 10(16), 3354-3363. PMCID:PMC4142076.

57) Cheng, Z, Al Zaki, A., Aspinwall, C., Tsourkas, A. (2014) Stabilized porous liposomes with encapsulated Gd-labeled dextran as highly efficient MRI contrast agents. Chemical Communications, 50(19), 2502-2504. PMCID:PMC3947407.

56) Al Zaki, A., Joh, D., Cheng, Z., de Barros, A.L., Kao, G.D., Dorsey, J.F., Tsourkas, A. (2014) Gold-loaded polymeric micelles for computed tomography–guided radiation therapy treatment and radiosensitization. ACS Nano, 8(1), 104-112. PMCID: PMC3906892.

55) Song*, Y., Zhang*, X., Huang, L., Behlke, M.A., Tsourkas, A. (2014) Real-time imaging of single engineered RNA transcripts in living cells using ratiometric bimolecular beacons. Journal of Visualized Experiments, Aug 6 (90). (*contributed equally).

54) Zhang, X., Zajac, A.L., Huang, L., Behlke, M.A., Tsourkas, A. (2014) Imaging the directed transport of single engineered RNA transcripts in real-time using ratiometric bimolecular beacons. PLoS One. 9(1), e85813. PMCID:PMC3893274.

53) Nwe, K., Huang, C-H., Tsourkas, A. (2013) Gadolinium-labeled glycol chitosan as a pH-responsive magnetic resonance imaging agent for detecting acidic tumor microenvironments. Journal of Medicinal Chemistry, 56(20), 7862-7869. PMCID:PMC3880625.

52) Dorsey, J.F., Sun, L., Joh, D.Y., Witztum, A., Kao, G.D., Alonso-Basanta, M., Avery, S., Hahn, S.M., Al Zaki, A., Tsourkas, A. (2013) Gold nanoparticles in radiation research: potential applications for imaging and radiosensitization. Translational Cancer Research, 2(4), 280-291. PMCID: PMC4241969.

51) Warden-Rothman, R., Caturegli, I., Popik, V., Tsourkas, A. (2013) Sortase-Tag Expressed Protein Ligation (STEPL): combining protein purification and site-specific bioconjugation into a single step. Analytical Chemistry, 85(22), 11090-11097. PMCID: PMC3843242.

50) Joh, D.Y., Kao, G.D., Murty, S., Stangl, M., Sun, L., Al Zaki, A., Xu, X., Tsourkas, A., Dorsey, J.F. (2013) Theranostic gold nanoparticles modified for durable systemic circulation effectively and safely enhances the radiation therapy of human sarcoma cells and tumors. Translational Oncology, 6(6), 722-731. PMCID:PMC3890707.

49) de Barros, A.L., Chacko, A.M., Mikitsh, J.L, Al Zaki, A., Saboury, B., Tsourkas, A., Alavi, A. (2013) Assessment of global cardiac uptake of radiolabeled iron oxide nanoparticles in apolipoprotein E-deficient mice: implications for imaging cardiovascular inflammation. Molecular Imaging and Biology, 16(3), 330-339. PMCID:PMC4016162.

48) Zhang, X., Song, Y., Shah, A.Y., Lekova, V., Raj, A., Huang, L., Behlke, M.A., Tsourkas, A. (2013) Quantitative Assessment of Ratiometric BiMolecular Beacons as a Tool for Imaging Single Engineered RNA Transcripts and Measuring Gene Expression in Living Cells. Nucleic Acids Research, 41(15), e152. PMCID: PMC3753654.

47) Joh, D.Y., Sun, L., Stangl, M., Al Zaki, A., Murty, S., Davis, J.J., Racharla, L., Bhang, D.H., Baumann, B.C., Alonso-Basanta, M., Ryeom, S.W., Kao, G.D., Tsourkas, A., Dorsey, J.F. (2013) Selective targeting of brain tumors with nanoparticle-induced radiosensitization. PLoS One, 8(4), e62425. PMCID: PMC3640092.

46) Huang, C-H., Tsourkas, A. (2013) Gd-based macromolecules and nanoparticles as magnetic resonance contrast agents for molecular imaging. Current Topics in Medicinal Chemistry, 13(4), 411-421. PMCID: PMC3761358.

45) Elias, D. R., Poloukhtine, A., Popik, V., Tsourkas, A. (2013) Effect of ligand density, receptor density, and nanoparticle size on cell targeting. Nanomedicine. 9(2), 194-201. PMCID: PMC3502720.

44) Cheng, Z., Al Zaki, A., Hui, J.Z., Muzykantov, V.R., Tsourkas, A. (2012) Multifunctional Nanoparticles: Cost versus benefit of adding targeting and imaging capabilities. Science, 338(6109), 903-910. PMCID: PMC3660151.

43) Huang, C-H., Nwe, K., Al Zaki, A., Brechbiel, M. Tsourkas, A. (2012) Biodegradable polydisulfide dendrimer nanoclusters as MRI contrast agents. ACS Nano, 6(11), 9416-9424. PMCID: PMC3508381.

42) Cheng, Z., Al Zaki, A., Hui, J.Z., Tsourkas, A. (2012) Simultaneous quantification of tumor uptake for targeted and non-targeted liposomes and their encapsulated contents by ICP-MS. Analytical Chemistry. 84(17), 7578-7582. PMCID: PMC3443955

41) de Barros, A.L., Tsourkas, A., Saboury, B., Cardoso, V.N., Alavi, A. (2012) Emerging role of radiolabeled nanoparticles as an effective diagnostic technique. European Journal of Nuclear Medicine and Molecular Imaging, 2(1), 39. PMCID: PMC3441881.

40) Czupryna, J., Tsourkas, A. (2012) Xanthine oxidase-generated hydrogen peroxide is a consequence, not a mediator of cell death. FEBS Journal, 279(5), 844-855.

39) Crayton*, S.H., Elias*, D.R., Al-Zaki, AW, Cheng, Z., Tsourkas, A. (2012) ICP-MS analysis of lanthanide-doped nanoparticles as a non-radiative, multiplex approach to quantify biodistribution.  Biomaterials, 33(5), 1509-1519. (*contributed equally). PMCID: PMC3237748.

38) Crayton, S.H., Tsourkas, A. (2011) pH-titratable superparamagnetic iron oxide for improved nanoparticle retention in acidic tumor microenvironments. ACS Nano, 5(12), 9592-9601. PMCID: PMC3246562.

37) Cheng, Z., Elias, D.R., Kamat, N.P., Johnston, E.D., Poloukhtine, A., Popik, V., Hammer, D.A., Tsourkas, A.  (2011) Improved tumor targeting of polymer-based nanovesicles using polymer-lipid blends.  Bioconjugate Chemistry, 2(11), 2021-2029. PMCID: PMC3197750.

36) Czupryna, J., Tsourkas, A. (2011) Firefly luciferase and RLuc8 exhibit differential sensitivity to oxidative stress in apoptotic cells.  PLoS One, 6(5), e20073. PMCID: PMC3094452

35) Thorek, D.L.J, Weisshaar C.L., Czupryna, J.C., Winkelstein B.A., Tsourkas A. (2011) SPIO-enhanced detection of transient cervical nerve root compression.  Molecular Imaging, 10(3), 206-214.

34) Elias, D.R., Cheng, Z., Tsourkas, A. (2010) An Intein-Mediated Site-Specific Click Bioconjugation Strategy for Improved Tumor Targeting of Nanoparticle Systems.  Small, 6(21), 2460-2468.  PMCID: PMC3019098

33) Chen, A., Davydenko, O., Behlke, M.A., Tsourkas, A. (2010) Ratiometric BiMolecular Beacons for the sensitive detection of RNA in single living cells.  Nucleic Acids Research, 38(14), e148.  PMCID: PMC2919734

32) Thorek, D.L.J., Tsao, P.Y., Arora, V., Zhou, L., Eisenberg, R.A., Tsourkas, A. (2010) In vivo multimodal imaging of B cell distribution and response to antibody immunotherapy in mice.  PLoS One, 5(5), e10655.  PMCID: PMC2871797

31) Cheng, Z., Chen, A., Lee, H.-Y., Tsourkas, A. (2010) Examination of folate-receptor targeted liposomes with encapsulated poly(2-propylacrylic acid) as a pH-responsive nanoplatform for cytosolic drug delivery.  Small, 6(13), 1398-1401.

30) Cheng, Z., Thorek, D.L.J., Tsourkas, A. (2010) Gd-conjugated dendrimer nanoclusters as a tumor targeted T1 magnetic resonance imaging contrast agent.  Angewandte Chemie International Edition, 49(2), 346-350.  PMCID: PMC2862691

29) Warden, R., Saunders, J., Tsourkas, A. (2009) Commentary: Two-photon imaging of multicellular tumor spheroids: a novel method for evaluating the efficacy of cdc25 inhibitors.  Cancer Biology and Therapy,  8(23), 2237-2239. 

28) Chen, A.K., Behlke, M.A., Tsourkas, A. (2009) Sub-cellular trafficking and functionality of 2’-O-methyl and 2’-O-methyl-phosphorothioate molecular beacons.  Nucleic Acids Research, 37(22), e149.  PMCID: PMC2794186

27) Chen, A.K., Tsourkas, A. (2009) Imaging RNA in living cells with molecular beacons: current perspective and challenges. Journal of Innovative Optical Health Sciences, 2(4), 315-324.

26) Cheng, Z., Thorek, D.L.J, Tsourkas, A. (2009) Porous polymersomes with encapsulated Gd-labeled dendrimers as highly efficient MRI contrast agents. Advanced Functional Materials, 19(23), 3753-3759.

25) Thorek, D.L.J., Elias, D.R, Tsourkas, A. (2009) Comparative analysis of nanoparticle-antibody conjugations: carbodiimide versus click chemistry. Molecular Imaging, 8(4), 221-229. 

24) Lu, J., Tsourkas, A. (2009) Imaging individual microRNAs in single mammalian cells in situ.  Nucleic Acids Research, 37(14), e100.  PMCID: PMC2724290

23) Bao, G., Rhee, W., Tsourkas, A. (2009) Fluorescent probes for live cell RNA detection.  Annual Reviews of Biomedical Engineering, 11, 25-47.  PMCID: PMC2734976

22) Chen, A.K., Cheng, Z., Behlke, M.A., Tsourkas, A. (2008) Assessing the sensitivity of commercially available fluorophores to the intracellular environment.  Analytical Chemistry, 80(19), 7437-7444.  PMCID: PMC2626168

21) Elias, D.R., Thorek, D.L.J., Chen, A.K., Czupryna, J., Tsourkas, A. (2008) In vivo imaging of cancer biomarkers using activatable molecular probes.  Cancer Biomarkers, 4(6), 287-305. 

20) Thorek, D.L.J., Tsourkas, A. (2008) Size, charge, and concentration dependent uptake of iron oxide nanoparticles by non-phagocytic cells: a comparative study of USPIO, SSPIO, and MPIO.  Biomaterials, 29(26), 3583-3590.  PMCID: PMC2518173

19) Chen, A.K., Behlke, M.A., Tsourkas, A. (2008) Efficient cytosolic delivery of molecular beacon conjugates and flow cytometric analysis of target RNA.  Nucleic Acids Research, 36(12), e69.  PMCID: PMC2475621

18) Cheng, Z., Tsourkas, A. (2008) Paramagnetic porous polymersomes. Langmuir, 24(15), 8169-8173.  PMCID: PMC2646750

17) Zhang, C.Y., Lu, J., Tsourkas, A. (2008) Iron chelator-based amplification strategy for improved targeting of transferrin receptor with SPIO.  Cancer Biology and Therapy, 7(6), 889-895. 

16) Chen, A.K., Behlke, M.A., Tsourkas, A. (2007) Avoiding false-positive signals with nuclease-vulnerable molecular beacons in single living cells.  Nucleic Acids Research, 35(16), e105.  PMCID: PMC2018645

15) Czupryna, J., Tsourkas, A. (2006) Commentary: Suicide gene delivery by calcium phosphate nanoparticles: a novel method of targeted therapy for gastric cancer.  Cancer Biology and Therapy, 5(12), 1691-1692. 

14) Thorek, D.L.J., Chen, A.K., Czupryna, J., Tsourkas, A. (2006) Superparamagnetic iron oxide nanoparticle probes for molecular imaging.  Annals of Biomedical Engineering, 34(1), 23-38. 

13) Tsourkas*, A., Shinde-Patil*, V., Wolley, A., Allport, J., Weissleder, R. (2005) In vivo imaging of activated endothelium using anti-VCAM-1 magnetooptical probes. Bioconjugate Chemistry, 16(3), 576-581 (*contributed equally). 

12) Tsourkas, A., Newton, G., Perez, J., Basilion, J., Weissleder, R. (2005) Detection of peroxidase/H2O2-mediated oxidation with enhanced yellow fluorescent protein.  Analytical Chemistry, 77(9), 2862-2867. 

11) Kelly*, K., Allport*, J., Tsourkas, A., Shinde-Patil, V., Josephson, L., Weissleder, R. (2005) Detection of vascular adhesion molecule-1 expression using a novel multimodal nanoparticle.  Circulation Research, 96, 327-336(*contributed equally). 

10) Tsourkas, A., Weissleder, R. (2004) Illuminating the dynamics of intracellular activity with ‘active’ molecular reporters.  Mechanics and Chemistry of Biosystems, 1(2), 133-145. 

9) Tsourkas, A., Hofstetter, O., Hofstetter, H., Weissleder, R., Josephson, L. (2004) Magnetic relaxation switch immunosensors detect enantiomeric impurities.  Angewandte Chemie International Edition, 43(18), 2395-2399. 

8) Perez, J., Simeone, F., Tsourkas, A., Josephson, L., Weissleder, R. (2004) Peroxidase substrate nanosensors for MR imaging.  Nano Letters, 4(1), 119-122.

7) Bao, G., Tsourkas, A., Santangelo, P. (2004) Engineering nanostructured probes for sensitive intracellular gene detection.  Mechanics and Chemistry of Biosystems, 1(1), 23-36. 

6) Santangelo, P., Nix, B., Tsourkas, A., Bao, G. (2004) Dual FRET molecular beacons for mRNA detection in living cells.  Nucleic Acids Research, 32(6), e57.  PMCID: PMC390379

5) Tsourkas, A., Behlke, M., Xu, Y., Bao, G. (2003) Spectroscopic features of dual fluorescence/luminescence resonance energy-transfer molecular beacons.  Analytical Chemistry, 75(15), 3697-3703. 

4) Tsourkas, A., Behlke, M., Rose, S., Bao, G. (2003) Hybridization kinetics and thermodynamics of molecular beacons.  Nucleic Acids Research, 31(4), 1319-1330.  PMCID: PMC150230

3) Tsourkas, A., Bao, G. (2002) Shedding light on health and disease using molecular beacons.  Briefings in Functional Genomics and Proteomics, 1(4), 1-13. 

2) Tsourkas, A., Behlke, M., Bao, G. (2002) Structure-function relationships of shared-stem and conventional molecular beacons.  Nucleic Acids Research, 30(19), 4208-4215.  PMCID: PMC140536

1) Tsourkas, A., Behlke, M., Bao, G. (2002) Hybridization of 2’-O-methyl and 2’-deoxy molecular beacons to RNA and DNA targets.  Nucleic Acids Research, 30(23), 5168-5174.  PMCID: PMC137950


Book Chapters

7) Bao, G, Wile, B., Tsourkas, A. (2013) Engineering nano-probes for live-cell imaging of gene expression In Nano and Cell Mechanics: Fundamentals and Frontiers, edited by Espinosa, H.D., Bao, G., Wiley.

6) Crayton, S.H., Chen, A.K., Cheng, Z., Tsourkas, A. (2011) Molecular Imaging In Comprehensive Biomaterials, edited by Ducheyne, P., Healy, K., Hutmacher, D., Kirkpatrick, J., Elsevier Inc.

5) Chen, A.K., Rhee, W.J., Bao, G., Tsourkas, A. (2010) Delivery of molecular beacons for live-cell imaging and analysis of RNA In Methods in Molecular Biology: Messages on the move: Techniques in RNA visualization, edited by Gerst, J.E., The Humana Press Inc.

4) Lu, J., Tsourkas, A. (2010) Quantification of miRNA abundance in single cells using locked nucleic acid-FISH and enzyme labeled fluorescence In Molecular Imaging: Methods and Protocols, edited by Shah, K., The Humana Press Inc.

3) Elias, A., Tsourkas, A. (2009) Imaging circulating cells and lymphoid tissues with iron oxide nanoparticles In Hematology American Society of Hematology Education Program, 720-726.

2) Tsourkas, A., Josephson, L. (2008) Magnetic Nanoparticles In Molecular Imaging: Principles and Practice, edited by Weissleder, R., Gambhir, S.S., Ross, B.D., Rehemtulla, A.  BC Decker Inc, Publisher.

1) Thorek, D.L.J., Czupryna, J., Chen, A.K., Tsourkas, A. (2006) Molecular imaging of cancer with superparamagnetic iron oxide nanoparticles In Cancer Imaging, edited by M.A. Hayat.  Elsevier Science/Academic Press.