Abstract:
The primary goal of this experiment was to accelerate the growth rate
of the K-12 strain of Escherichia coli through the addition of nutrients
to the media of the standard experiment (10.0 ? 0.1g bacto-tryptone and
5.0 ? 0.1g yeast extract). [11] This was accomplished by the addition of
glucose, a carbon source, in the form of corn syrup to the control media
while maintaining constant environmental growth conditions. Additionally,
using scientifically published paper [9] a 35.1% increase in growth rate
constant was predicted. One control and two experimental trials were
then performed. The growth rate constant for the control experiment
was 0.0188 min-1 ? 4.05%. The average growth rate constant for the
two experimental trials conducted was 0.0239 ? min-1 ? 6.05% thereby yielding
a 27.13% increase in the growth rate constant from that of the control
experiment. This percentage increase deviated from the predicted
increase by 22.7%.