Inhibition of Growth of E. coli:Effects of Oxygen Concentration in Solution in Media

Class: BE210
Group: W1
Members: Yvonne Chen, LiKang Chin, Melanie Gnazzo, Jeffrey Levisman
Date: May 7, 1999

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Abstract:
E. coli growth was tested in a controlled environment at different concentrations of oxygen of 5% O2 and 9% O2 in the media, and was compared to the growth at 21% O2. It was found that E. coli growth was inhibited and showed anaerobic growth at 5% O2 with a growth rate of 0.0062 min-1 in comparison to the 21% O2 with a growth rate of 0.0097 min-1.  At 9% O2, the growth rate was comparable to the growth rate at the 21% O2 control of that trial, both at 0.0081 min-1, and thus aerobic growth was concluded to occur at this concentration of oxygen.  The threshold of anaerobic to aerobic growth was found to take place between 5% O2 and 9% O2.