Class: BE210
Group: R4
Members:   FACILITATOR………………………………..JANELLE VILLIERS

                    TIME & TASK KEEPER…………………..…JARED SHOEMAKER

                     SCRIBE………………………………………..NAMRATA CHOUDHURY

                     PRESENTER………………………………….PHILLIP MATSUNAGA &  GIE NA YU

Date: May, 2001

Full Text

Abstract:
Saccharomyces cerevisiae (baker’s yeast) was grown aerobically in the PENNCELL apparatus.  Death rate constants were calculated at different temperatures using the plot of absorbance vs. time.  The death rate constants and corresponding 95% confidence intervals for 44ºC, 46ºC, and 48ºC were 4.26E-3 ± 0.438E-3 min-1, 9.14E-3 ± 0.665E-3 min-1, and 36.1E-3 ± 5.409E-3 min-1 respectively.  The death activation energy Ed could then be calculated from the plot of ln kd versus 1/RT where kd is the death rate constant and T is temperature in Kelvin.  The calculated value of Ed was 108.058 kcal/mol with a 95% confidence interval of 231.466 kcal/mol.  This value was compared to the growth activation energy of aerobically grown yeast and death activation energy of anaerobically grown yeast.  When the large confidence interval was disregarded, the obtained Ed seemed reasonable.