Extraction and Determination of Visible Spectrum of Water Soluble Vegetable Pigments


Class: BE210
Group: W6
Members:       FACILITATOR………………………..…………Uduak Umoh

                        TIME & TASK KEEPER…………………………John Pan

                         SCRIBE…………………………………………..Anupam Gupta

                         PRESENTER…………………………………….Courtney Morgan

Date: May, 2001

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Abstract:
The optimal extraction method of the biological pigment, betanin was determined to be heating the beets in aqueous solution.  Also, there exists a linear dependence of the visible spectra on pH.  The linear relationship is that for organically grown beets (lmax)=1.281(pH)+531.34 and for non-organically grown beets (lmax)=1.878(pH)+527.63; however there are statistically insignificantly different from each other according to their respective 95% confidence intervals of 0.622-1.940 and 1.484-2.273, respectively.  In addition, the relationship between absorbance and molar extinction coefficient*concentration was determined to be (Absorbance)=0.0189(%Working Solution Concentration) + 0.0027 with the slope having a 95% confidence interval of 0.01882-0.01892.  Lastly, no pKa values could be determined from the titration curve of betanin.