Medium Modification to Determine a Cost-Efficient Growth Environment for Yeast


Class: BE210
Group: T6
Members:

Chen
Goyal
Puri
Shin

Date: April 2004

Full Text

Abstract:

Many biotechnological processes used in industry bank on the reliable and cost-effect production of Saccharomyces cerevisiae, commonly known as Baker's Yeast. Yeast can grow in the presence of just sugars, which provide a source of glucose. However, yeast grows much faster when provided with proteins as well as biotin, trace metals, and salts, which are needed for growth. Proteins, which serve to provide amino acids as well as perform enzymatic activities, can be supplied through peptone—partially digested proteins. Yeast extract is obtained by the autolysis of yeast cells, and therefore, provides growing yeast with all the necessary components, including biotin, trace metals and salts, as well as proteins.

Although yeast has been proven to grow effectively with just dextrose, previous experimentation has suggested that the yeast extract has the most significant effect on the reproduction of yeast. “The addition of protein and yeast cell extract hydrolysates allow faster growth so that during exponential or log-phase growth, the cells divide every 90 minutes.”  Moreover, during the lag phase secondary metabolites required for growth are produced. Since the yeast extract contains ready-made secondary metabolites it reduces the time required for the lag phase to transition into the log phase.

Cost-efficiency of yeast growth is relevant in several sectors of industry. Yeast is used as a fermenting agent in bread-making, wine-making, the production of cheese and yogurt, and the production of various other foods that require fermentation. Besides fermentation, yeast can be used to create yeast extract spread for direct consumption. It is in the best interest of industrial manufacturers to determine the specific combinations of yeast extract, peptone, and dextrose that produce the desired results according to each manufacturer’s financial situation and/or demand for their product.