The Effect of pH Buffering on Yeast (Saccharomyces cerevisiae) Growth


Class: BE210
Group: W6
Members:

Harris
Weisman
Chung
Kwong
Pacheco

Date: April 2004

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Abstract:

Many factors influence the rate of yeast growth including temperature, nutrient supply and pH. To study the effect of pH buffering on yeast growth, it is necessary to attempt to eliminate potential environmental variables that may arise by holding temperature, nutrient supply and overall laboratory conditions constant throughout the experiment. In order to create a buffer that holds the pH steady in the optimal pH range for yeast growth, the optimal range must be determined.  Research has shown that there is no agreed upon pH value that is optimal for yeast growth, rather the optimal range runs from pH 4 to pH 8 depending on the source , making it clear that the optimal pH is unique to each experiment. A by-product of yeast growth is hydrogen ions, which make the pH of the growth medium more acidic as they are pumped out of the cells.