Effect of Heat on Muscle Protein Detection by Gel Electrophoresis

Diseased and Normal Cardiac Muscle Comparison Using Electrophoresis

Name: Vivian Shaw

Class: BE 210

Hypothesis:

Myosin, a common protein in muscle tissue, will be more abundant in the cardiac muscles of cardiac muscle without any major problems than in the cardiac muscle of a person with dilated cardiomyopathy.

Objectives and Aims:

Gain knowledge of electrophoresis, an experimental technique used to separate proteins on the basis of molecular weight or electrical charge.
To isolate, identify, and quantify protein in mammalian muscle using electrophoresis
To determine the effects upon the concentration of certain proteins in cardiac muscle of dilated cardiomyopathy