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The laboratory is located in the Towne Building, 3rd floor, room 390, microscopy suite. Towne is located between 33rd and 34th streets, directly across from Franklin Field. Our mailing address is: Department of Bioengineering, 120 Hayden Hall, 3320 Smith Walk, Philadelphia, PA 19104-6392. |
Click here to open a map to the Towne Building: 
Click on this link for maps of Penn: Maps of Penn Campus
| Answer: | The lab phone number is | 215-573-5191 |
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Our fax number is |
215-573-2071 |
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Contact Gladys Gray Lawrence, Co-Director at 215-573-5191 or by email at ggb@seas.upenn.edu. Also, read our Web page on Scheduling an Appointment. |
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We recommend that all investigators copy their data to a CD-ROM at the time of their confocal session. We usually can supply CD-ROMs, but we recommend that each user supply their own. Transfer over the network via FTP from a password-protected account can also be arranged. |
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Remember: BioRad PIC files are NOT Macintosh PIC files!
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For the PC: (Win 95, 98 and NT) use Confocal Assistant, free from BioRad (ftp://ftp.genetics.bio-rad.com/Public/confocal/cas/), or from our file server. Confocal assistant duplicates most functions of Lasersharp with the exception of morphometry. Shareware from Irfanview (www.irfanview.com) also works well to open and manipulate PIC files. |
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For the Mac: NIH Image and associated macros can be obtained from: (http://rsb.info.nih.gov/nih-image/). Graphic Converter is a shareware program from Lemke Software (www.lemkesoft.com) that some people prefer. |
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Confocal Assistant is rather quirky, if merge will not recognize files, try moving them to the same directory where CAS.EXE resides and try again. |
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A confocal microscope is merely a detector of fluorescence intensity. Data is acquired as intensity values at points within the sample . Therefore, by design, all files acquired on a confocal microscope are acquired as grayscale. However, since the channel from which the intensity measurements were made is known, the instrument - using the Lasersharp software - can reassemble a multichannel image providing appropriate colors for each channel. Remember though, any color attributed to a particular channel is artificial - it is "pseudocolor". If you are working on your own computer and want to color an image with Confocal Assistant, do the following: 1. Open a file. 2. Click on Image. 3. Click on Lookup Table and choose the color you would like to see. Note: If you plan to merge channels DO NOT overwrite your original data with a pseudocolored (RGB) image. Merge only works on grayscale images. |
| Answer: | Director: Susan Margulies, Ph.D., Professor in Bioengineering. | ||
| Phone: 215-898-0882 | Email: margulie@seas.upenn.edu | ||
| Co-Director: Gladys Gray Lawrence., Senior Research Scientist | |||
| Phone: 215-573-5191 | Email: ggb@seas.upenn.edu | ||
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Fax for both: 215-573-2071 |
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Answer: Available hours vary according to the users group. All use is by prior appointment only.
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