Click on a heading to go to that section.

This page may be searched by clicking using the find function of your browser.

Books

Mounting Media

Confocal Microscopy

Fixatives

2-photon Microscopy

Nuclear Staining

Fluorophore Usage & Data

Calcium Ratio Imaging

Antifade Reagents

Note: The body of literature for most of these areas is immense and growing. What follows are selected references we have found useful in our studies.

Books: Broad Technical Focus

Beltz, B.S. and Burd, G.D. (1989). Immunocytochemical Techniques: Principles and Practice. Blackwell Scientific Publications, Inc., Cambridge, MA.

Haugland, R. P. (1992). Handbook of Fluorescent Probes and Research Chemicals. Molecular Probes Inc., Eugene, OR.

Matsumoto, B., ed. (1993). "Cell biological applications of confocal microscopy." Academic Press. San Diego, California.

Pawley, J.B. (ed.) (1990). Handbook of Biological Confocal Microscopy. Plenum Press, New York, NY.

Wang, Y.L. and Taylor, D.L. (ed.) (1989). Fluorescence Microscopy of Living Cells in Culture. Part A: Fluorescent Analogs, Labeling Cells and Basic Microscopy. Methods in Cell Biology 29. Academic Press, San Diego, CA.

Nuclear Staining:

Smith PJ, Blunt N, et. al.(2000). Characteristics of a novel deep red/infrared fluorescent cell-permeant DNA probe, DRAQ5, in intact human cells analyzed by flow cytometry, confocal and multiphoton microscopy. Cytometry. 40(4):280-91.

Natalia G et al., (1985). Application of 7-amino-actinomycin D for the fluorescence microscopical analysis of DNA in cells and polytene chromosomes. Histochemical Journal 17, 131-142.  

Matsuzaki T., Suzuki T., Fujikura K., and Takata K.  (1997).  Nuclear staining for laser confocal microscopy.   Acta Histochem. Cytochem.  30, 309-314.

Valente et al. (1998). Propidium Iodide for Nuclear staining. Plant Science 134 (207-315).

Calcium Ratio Imaging:

Propagating Intercellular Calcium Waves in Airway Epithelia Are Mediated by Bilateral (Luminal and Serosal) 5' Nucleotide Release Madge, S. L. pp. 294 Pediatric Pulmonology -Supplement-, (19), 1999

Photophysics of the fluorescent Ca2+ indicator fura-2 Bergh, Viviane; Boens, Noešl; Schryver, Frans. C.; Ameloot, Marcel; Steels, Paul; Gallay, Jacques; Vincent, Michel; Kowalczyk, Andrzej pp. 1110-1119

R.B. Silver. (1998) Ratio Imaging: Practical Considerations for Measuring Intracellular Calcium and pH in Living Tissue. Meth Cell Biol 56, 237.

G. Grynkiewicz, M. Poenie, R.Y. Tsien. (1985). A New Generation of Ca2+ Indicators with Greatly Improved Fluorescence Properties.  J Biol Chem 260, 3440.

M. Wahl, M.J. Lucherini, E. Gruenstein. (1990). Intracellular Ca2+ Measurement with Indo-1 in Substrate-Attached Cells: Advantages and Special Considerations. Cell Calcium 11, 487.

Gasalla-Herraiz,J., Rhee,S. & Isales,C.M. (1995). Calcium-sensitive probes for the measurement of intracellular calcium: Effects of buffer system and magnesium concentration. Biochem.Biophys.Res.Commun. 214, 373-388.

2-Photon Microscopy:

Potter, S.M., Pine, J., Fraser, S.E. Neural transplant staining with DiI and vital imaging by 2-photon laser-scanning microscopy. (1996) Scanning Microscopy - Supplement;10:189-99.

Montrose, M.H., Imaging drug therapy and pH in gastrointestinal tissue with confocal and two-photon microscopy. (1998) Scanning; 20(3):148-9.

Denk, W., Svoboda, K. Photon upmanship: Why multiphoton imaging is more than a gimmick. (1997). Neuron 18(3). 351-357.

Svoboda, K., Denk, W., Kleinfeld, D., Tank, D.W. In vivo dendritic calcium dynamics in neocortical pyramidal neurons. (1997). Nature (London) 385(6612). 161-165.

Maiti, S.,  Shear, J.B., Williams, R.M., Zipfel, W.R., Webb, W.W. Measuring serotonin distribution in live cells with three-photon excitation. Science 1997 Jan 24;275(5299):480-1.

Shear, J.B.,  Xu, C., Webb, W.W. Multiphoton-excited visible emission by serotonin solutions. Photochemistry & Photobiology (1997). Jun;65(6):931-6.

Kohler, R.H.,  Cao, J., Zipfel, W.R., Webb, W.W., Hanson, M.R. Exchange of protein molecules through connections between higher plant plastids. (1997) Science Jun 27;276(5321):2039-42.

 

Confocal Microscopy:

Brelje TC, Wessendorf MW, Sorenson RL.. (2002) Multicolor laser scanning confocal immunofluorescence microscopy: Practical application and limitations. Methods in Cell Biology. 70:165-244.

Smith, C.L., Zaal, K.J., Lippincott-Schwartz, J., Looking at the bright side of photobleaching: analysis of intracellular protein dynamics by confocal microscopy.(1998). Scanning, 20(3):147-8.

Fluorophore Data and Usage

Use of DiI/DiO:
Probing the Lateral Organization and Dynamics of Membranes. D.E. Wolf. Spectroscopic Membrane Probes, Vol. I, L.M. Loew, Ed., pp. 193-220 (1988) PN27292.

Iontophoretic Dye Labeling of Embryonic Cells. S.E. Fraser. Meth Cell Biol 51, 147 (1996).

Novel E-Cadherin-Mediated Adhesion in Peripheral Nerve: Schwann Cell Architecture is Stabilized by Autotypic Adherens Junctions. A.M. Fannon, et al. J Cell Biol 129, 189 (1995).

Regulation of C-Cadherin Function during Activin Induced Morphogenesis of Xenopus Animal Caps. W.M. Brieher, B.M. Gumbiner. J Cell Biol 126, 519 (1994).

The Use of the Lipophilic Fluorochrome CM-DiI for Tracking the Migration of Lymphocytes. W. Andrade, et al. J Immunol Meth 194, 181 (1996).


BODIPY 558/568:
Motility and Cytoskeletal Organization of Migrating Cerebellar Granule Neurons. R.J. Rivas, M.E. Hatten. J Neurosci 15, 981 (1995).

How an Actin Network Might Cause Fountain Streaming and Nuclear Migration in the Syncytial Drosophila Embryo. G. von Dassow, G. Schubiger. J Cell Biol 127, 1637 (1994).


Oregon Green(r) 488 phalloidin:
ADP-Ribosylation Factor 6 Regulates a Novel Plasma Membrane Recycling Pathway." H. Radhakrishna, J.G. Donaldson. J Cell Biol 139, 49 (1997).

Actin-Binding Verprolin is a Polarity Development Protein Required for the Morphogenesis and Function of the Yeast Actin Cytoskeleton." G. Vaduva, N.C. Martin, A.K. Hopper. (1997). J Cell Biol 139, 1821 .


Hoechst H-1398:
Cooperative Interactions between the Central Spindle and Contractile Ring during Drosophila Cytokinesis. (1998). M.G. Giansanti, et al. Genes Devel 12, 396.

Mitochondrial Free Radical Signal in Ceramide-Dependent Apoptosis: A Putative Mechanism for Neuronal Death in Parkinson's Disease." V. France-Lanord, et al. J Neurochem 69, 1612 (1997).

"Fluorescence Spectra of DNA Dyes Measured in a Flow Cytometer." C.L. Asbury, et al. Cytometry 24, 234 (1996).

Bone Morphogenetic Proteins Promote Astroglial Lineage Commitment by Mammalian Subventricular Zone Progenitor Cells." R.E. Gross, et al. Neuron 17, 595 (1996)

 

Antifade Reagents:

Bock G., Hilchenbach M., Schauenstein K. and Wick, G. Photometric analysis of antifading reagents with laser and conventional illumination sources. J. Histochem. & Cytochem. 33(7):699-705, 1985.

Cytometry 19:177-182 (1995): "Analysis of antifading reagents for fluorescence microscopy."

Johnson G.D. and De C Nogueira Araujo G.M. A simple method for reducing the fading of immunofluorescence during microscopy. J. Immunol. Methods. 43:349, 1981.

Johnson G.D., Davidson R.S., McNamee K.C., Russell G., Goodwin D., and Holborow E.J. Fading of Immunofluorescence during microscopy: a study of the phenomenon and its remedy. J. Immunol. Methods. 55:231, 1982.

Krenik K.D., Kephart G.M., Offord K.P., Dunnette S.L. and Gleich, G.J. Comparison of antifading reagents used in immunofluorescence. J. Immunol. Methods. 117:91-7, 1989.

Longin A., Souchier C., French M., Bryon PA. Comparison of anti-fading agents used in fluorescence microscopy: image analysis and laser confocal microscopy study. J. Histochem. & Cytochem. 41(12):1833-40, 1993 Dec