 |
Figure 1:
Time-lapse sequence of neural processes translating a GFP construct
in response to agonist - in the absence of cell bodies. (Single
frame). Images by 2-photon excitation at 800nm, 120fs. Christy
Job, James Sanzo and James Eberwine. |
 |
Figure 2: Cultured
rat-brain slice culture labeled with Calcein Green and propidium iodide.
Theresa Lusardi, Mike Deridder, David Meaney and James Sanzo. |
 |
Figure 3: Stretched
neurons. John Wolf, Doug Smith and James Sanzo. |
 |
Figure 4: Cultured
type II pneumocytes labeled with Fluo-4. DIC- Nomarski image with
fluorescence overlay. Michele Hawk and James Sanzo. |
 |
Figure 5: Neural
cells grown out from the edge of a rat brain slice culture. Theresa Lusardi, David Meaney and
James Sanzo. |
 |
Figure 6: Neural
cells grown out from the edge of a rat brain slice culture. Rendered
3-D image with perspective. |
 |
Figure 7: Viewer
quiz: Can you identify this nuclear phenomenon? |
 |
Figure 8: Cultured
endothelial cells labeled with Alexa-488 phalloidin, Mitotracker Red,
and DAPI. Imaged with a combination of single photon and 2-photon
techniques. Maximum-intensity projection from partial stack. |
 |
Figure 9: Urethane
foam. Imaged in fluorescence mode for a commercial client. |
 |
Figure 10: Rat-1
cells transfected with insulin receptors. Insulin is red, receptors
are blue and F-actin is green. |
 |
Figure 11: Canine
kidney, stained with eosin, imaged by 2-photon microscopy. ~70
microns below the surface. |
 |
Figure 12: Human
retina stained with a photo receptor-specific protein (eap7) and
DAPI. Imaged with a combination of single photon and 2-photon
techniques. Eric Pierce, Ann Milam, James Sanzo. |
 |
Figure 13: Human
retina stained with a cone-specific protein, rhodopsin and
DAPI. Imaged with a combination of single photon and 2-photon
techniques. John Sinoj, Petros, James Sanzo. |
 |
Figure 14: Live
tumor
vasculature stained with a FITC-lectin, costained with Hoechst
33342.
2-photon imaging. Mike Gee, William Lee, James Sanzo. |
 |
Figure 15: Herpes
simplex virus (green) invading cells in culture. Combined single and
2-photon imaging. F-actin is red (phalloidin), Nuclei are blue
(DAPI). Gary Cohen,
Isabelle Baribaud, James Sanzo. |
 |
Figure 16: Di-I
stained lipid uptake. Vesicles loaded with DI-I stained lipids show
as red in this combined fluorescence-DIC image. Hui Li, Briton
Chance, James Sanzo. |
 |
Figure 17:
Maximum-intensity projection of retrovirally transduced 3T3 cells.
Green = actin, Red = nucleolar expression of able. Jason
Wertheim, Dan Hammer, James Sanzo. |
 |
Figure 18: Chick
limb, 780 microns thick. Imaged with 10x plan-apo objective lens.
Maximum-intensity reconstruction rotated 5° right of perpendicular.
Green =
vimentin, red = collagen I. Lilly Wong, James Sanzo. |
 |
Figure 19: Mouse retina stained with a
photo receptor specific protein and DAPI. Imaged with single photon
(Texas Red) and 2-photon (DAPI) techniques. John Sinoj, Petros, James Sanzo. |
 |
Figure 20:
Lysozyme crystal imaged by reflection of 488nm line. Reconstructed
from a 155 plane stack. Asif Mustafa, Donald Voet, James Sanzo. |
 |
Figure 21:
Neuronal cells plated onto nitride passivated silicon.
Simultaneously imaged by reflection mode (gray = nitride) and
fluorescence mode (green = calcein). |
 |
Figure 22:
Neuronal cells plated onto nitride passivated silicon.
Simultaneously imaged by reflection mode (dark = nitride, gold =
traces, blue = electrode) and
fluorescence mode (green = calcein in live cells). |
 |
Figure 23:
Filaments of auto-antigen Ro (green = GFP-Ro) as seen during
time-lapse sequence leading to apoptosis. Images by 2-photon
microscopy + DIC. |
 |
Figure 24:
Abnormal live vasculature in induced mouse tumor. Stained with a
FITC-tomato lectin, costained with Hoechst
33342.
2-photon imaging. |
 |
Figure 25:
Teased muscle fibers showing partial expression of GFP-actin. |
 |
Figure 26: Herpes
simplex virus (green) invading cells in culture. Combined single and
2-photon imaging. F-actin is red (phalloidin), Nuclei are blue
(DAPI). Max intensity projection of full stack. |
 |
Figure 27:
Cytokeratin-19 (green) and desmoplakin (red). Fixed immunostained
cells. |
 |
Figure 28: Human
retina stained for a rod-specific protein and
DAPI. Imaged with a combination of single photon and 2-photon
techniques |
|
|
