Jinzhao SongJinzhao Song Penn Engineering

Research Associate in Prof. Haim Bau's Micro- & Nano-fluidics Lab

Contact: songjinz@seas.upenn.edu

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Research Interests: Point of Care Diagnostics, Companion Genetic Diagnosis





My Publications:

1. Michael G. Mauk*, Changchun Liu, Qiu X, Chen D, Jinzhao Song, Haim H. Bau. Microfluidic "Pouch" Chips for Immunoassays and Nucleic Acid Amplification Tests. Methods Mol Biol 1572, 467-488 (2017).

2. Jinzhao Song, Changchun Liu, Michael G. Mauk, Shelley C. Rankin, Robert M. Greenberg, and Haim H. Bau*. Two-stage isothermal enzymatic amplification for concurrent multiplex molecular detection. Clinical Chemistry, 63, 714-722 (2017).

3. Michael G. Mauk, Jinzhao Song, Haim H Bau, Robert Gross, Frederic Bushman, Ronald Collman, and Changchun Liu*. Miniaturized Devices for Point of Care Molecular Detection of HIV. Lab Chip, published on line, DOI: 10.1039/C6LC01239F (2016).

4. Jinzhao Song, Michael Mauk, Brent Hackett, Sara Cherry, Haim H. Bau and Changchun Liu*. Instrument-Free Point-of- Care Molecular Detection of Zika Virus. Anal. Chem., 88 (14), 7289-7294 (2016).

5. Changchun Liu*, Shih-Chuan Liao, Jinzhao Song, Michael G. Mauk, Xuanwen Li, Gaoxiang Wu, Dengteng Ge, Robert M. Greenberg, Shu Yang, and Haim H. Bau. A high-efficiency superhydrophobic plasma separator. Lab Chip 16, 553-560 (2016).

6. Shih-Chuan Liao, Jing Peng, Michael G. Mauk, Sita Awasthi, Jinzhao Song, Harvey Friedman, Haim H. Bau, Changchun Liu*. Smart Cup: A Minimally-Instrumented, Smartphone-Based Point-of- Care Molecular Diagnostic Device. Sens Actuators B Chem 229, 232-238 (2016).

7. Michael G. Mauk, Jinzhao Song, Yubing Tong, Haim H. Bau, and Changchun Liu*. Translating Nucleic Acid Amplification Assays to the Microscale: Lab on a Chip for Point-of- Care Molecular Diagnostics. Current Analytical Chemistry, 12, DOI: 10.2174/1573411012666151020212121 (2016).

8. Jinzhao Song, Changchun Liu, Swarna Bais, Michael G. Mauk, Haim H. Bau, Robert M. Greenberg*. Molecular Detection of Schistosome Infections with a Disposable Microfluidic Cassette. PLos Neglect Trop D. 9(12), doi: ARTN e0004318 (2015).

9. Michael G. Mauk *, Changchun Liu, Jinzhao Song, and Haim H. Bau. Integrated Microfluidic Nucleic Acid Isolation, Isothermal Amplification, and Amplicon Quantification. Microarrays, 4, 474-489 (2015).

10. Karen E. Ocwieja, Scott Sherrill-Mix, Changchun Liu, Jinzhao Song, Haim H. Bau, Frederic D. Bushman*. A Reverse Transcription Loop-Mediated Isothermal Amplification Assay Optimized to Detect Multiple HIV Subtypes. PLoS ONE 10(2): e0117852 (2015).

11. Millicent Lin, Jie-Fu Chen, Yi-Tsung Lu, Yang Zhang, Jinzhao Song, Shuang Hou,* Zunfu Ke,* and Hsian-Rong Tseng*. Nanostructures Embedded Microchips for Detection, Isolation, and Characterization of Circulating Tumor Cells. Acc. Chem. Res. 47, 2941-2950 (2014).

12. Bing Wang, Jinzhao Song, Huanxiang Yuan, Chenyao Nie, Fengting Lv, Libing Liu, Shu Wang*. Multicellular Assembly and Light-Regulation of Cell–Cell Communication by Conjugated Polymer Materials. Adv. Mater. 26, 2371-2375 (2014).

13. Jiangyan Zhang, Baoling Xing, Jinzhao Song, Feng Zhang, Chenyao Nie, Lian Jiao, Libing Liu, Fengting Lv, Shu Wang*. Associated Analysis of DNA Methylation for Cancer Detection Using CCP-Based FRET Technique. Anal. Chem. 86, 346–350 (2014).

14. Jinzhao Song, Jiangyan Zhang, Fengting Lv*, Yongqiang Cheng, Bing Wang, Liheng Feng, Libing Liu, Shu Wang*. Multiplex detection of DNA mutations by fluorescence fingerprint spectrum technique. Angew. Chem. Int. Ed. 52, 13020- 13023 (2013).

15. Baoling Xing, Jinzhao Song (co-first author), Sumei Ge, Zhenghua Tang, Menglu Liu, Qiong Yang*, Fengting Lv, Libing Liu, Shu Wang*. Multiplex detection of KRAS and BRAF mutations using cationic conjugated polymers. Chin. Sci. Bull. 58, 873-878 (2013).

16. Hui Chong, Chunlei Zhu, Jinzhao Song, Liheng Feng, Qiong Yang*, Libing Liu, Fengting Lv, Shu Wang*. Preparation and optical property of new fluorescent nanoparticles. Macromol. Rapid Commun. 34, 736-742 (2013).

17. Jinzhao Song, Fengting Lv*, Gaomai Yang, Libing Liu, Qiong Yang, Shu Wang*. Aptamer-based polymerase chain reaction for ultrasensitive cell detection. Chem. Commun. 48, 7465-7467 (2012).

18. Jinzhao Song, Qiong Yang*, Fengting Lv, Libing Liu, Shu Wang*. Visual detection of DNA mutation using multicolor fluorescent coding. ACS Appl. Mater. Interfaces 4, 2885-2890 (2012).

19. Jinzhao Song, Zhengping Li*, Yongqiang Cheng, Chenghui Liu. Self-aggregation of oligonucleotide-functionalized gold nanoparticles and its applications for highly sensitive detection of DNA. Chem. Commun. 46, 5548-5550 (2010).

20. Zhengping Li*, Yongshan Fan, Jinzhao Song, Chenghui Liu. Direct Visualization of MicroRNAs Based on Non-cross- linking gold nanoparticle aggregation. Progress on Post-genome Technologies: Proceedings of the 6th International Forum of Post-genome Technologies (2009).

My Research:

Over the past 10 years, I have been focusing on development of next generation point-of-care diagnostic system in the following aspects:

• Development of Next Generation Point-of-Care Diagnostic System Based on Microfluidic Chip and Nucleic Acid Isothermal Amplification.

Key results include: (1) Designed a point-of-care device and firstly developed specific Loop-Mediated Isothermal Amplification (LAMP) primer set for molecular detection of Zika Virus. (2) Established a two-stage isothermal amplification process, dubbed RAMP, achieving high level of multiplexing (16, more is feasible) with order of magnitude higher sensitivity than single stage assays, high speed (<40 min), and high tolerance to inhibitors. Designed a corresponding chip for automatically distributing first-stage products to second-stage reactor. (3) Created a disposable microfluidic cassette for molecular detection of schistosome infections.

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(1) Jinzhao Song, Michael Mauk, Brent Hackett, Sara Cherry, Haim H. Bau and Changchun Liu*. Instrument-Free Point-of-Care Molecular Detection of Zika Virus. Anal. Chem., 88 (14), 7289-7294 (2016).

(2) Jinzhao Song, Changchun Liu, Michael G. Mauk, Shelley C. Rankin, Robert M. Greenberg, and Haim H. Bau*. Two-stage isothermal enzymatic amplification for concurrent multiplex molecular detection. Clinical Chemistry, 63, 714-722 (2017).

(3) Jinzhao Song, Changchun Liu, Swarna Bais, Michael G. Mauk, Haim H. Bau, Robert M. Greenberg*. Molecular Detection of Schistosome Infections with a Disposable Microfluidic Cassette. PLos Neglect Trop D. 9(12), doi: ARTN e0004318 (2015).

• Application Research of Novel Materials (Conjugated Polymers and Nanoparticles) in Next Generation Point-of-Care Diagnostic System.

My contributions are: (1) Developed a series of methods for gene mutation detection using water-soluble cationic conjugated polymers. (2) Established a new system for sensitive and selective detection of circulating tumor cells by combining Aptamer-immuno-PCR and signal amplification of conjugated polymers. (3) Discovered a new self-aggregation phenomenon of single stranded (ss) DNA-modified gold nanoparticles (ssDNA-GNPs), which was used for DNA visualized detection. In this work, I also clarified the mechanism of non-crosslinking aggregation of gold nanoparticles that is confounded in academia for a long time.

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(1) Jinzhao Song, Jiangyan Zhang, Fengting Lv*, Yongqiang Cheng, Bing Wang, Liheng Feng, Libing Liu, Shu Wang*. Multiplex detection of DNA mutations by fluorescence fingerprint spectrum technique. Angew. Chem. Int. Ed. 52, 13020-13023 (2013).

(2) Jinzhao Song, Qiong Yang*, Fengting Lv, Libing Liu, Shu Wang*. Visual detection of DNA mutation using multicolor fluorescent coding. ACS Appl. Mater. Interfaces 4, 2885-2890 (2012).

(3) Jinzhao Song, Fengting Lv*, Gaomai Yang, Libing Liu, Qiong Yang, Shu Wang*. Aptamer-based polymerase chain reaction for ultrasensitive cell detection. Chem. Commun. 48, 7465-7467 (2012).

(4) Jinzhao Song, Zhengping Li*, Yongqiang Cheng, Chenghui Liu. Self-aggregation of oligonucleotide-functionalized gold nanoparticles and its applications for highly sensitive detection of DNA. Chem. Commun. 46, 5548-5550 (2010).