Class: BE-309
Group: M2
Members: Dominic Mangiardi, Alisa Plesco, Lisa Romito, David Thakker
Date: Fall 1998
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Abstract:
In this experiment, sodium dithionite was used as a reducing agent in a Sephadex G-25 fine gel column to convert methemoglobin to hemoglobin. Qualitative indication of reduction was seen as the brown colored methemoglobin passed through the column and exited as a red eluant. Quantitative comparison was shown using a spectrophotometer. The experimental absorbance spectrum obtained for HbO2 was shown to agree within 3.3% of the literature absorbance spectrum.