some journals


Click here for a complete publication list. [pubmed]

Stem Cells, Cancer, Nucleus, Matrix, & Mechanobiology
'Marker of Self' Immunobiology and Cell Membrane Dynamics
Materials Biology and Molecular Mechanics
Assemblies - Polymersomes, Filomicelles, & Molecular Dynamics


Stem Cells, Cancer, Nuclei, Extracellular Matrix, & Mechanobiology

J. Irianto, Y. Xia, C.R. Pfeifer, A. Athirasala, J. Ji, C. Alvey, M. Tewari, R.R. Bennett, S.M. Harding, A.J. Liu, R.A. Greenberg, and D.E. Discher. DNA damage follows repair factor depletion and portends genome variation in cancer cells after pore migration.

Current Biology 27, 210–223 (2017). [pdf]

S.M. Harding, J.L. Benci, J. Irianto, D.E. Discher, A.J. Minn, R.A. Greenberg. Mitotic progression following DNA damage enables pattern recognition within micronuclei.

Nature 23470 (2017).

J. Irianto, Y. Xia, C.R. Pfeifer, R.A. Greenberg, and D.E. Discher. As a nucleus enters a small pore, chromatin stretches and maintains integrity even with DNA breaks.

Biophysical JournalBiophysical Letter for Special Issue on Chromatin Biophysics  

112: 446-449 (2017). [pdf]

J. Irianto, C.R. Pfeifer, R.R. Bennett, Y. Xia, I.L. Ivanovska, A.J. Liu, R.A. Greenberg, and D.E. Discher. Nuclear constriction segregates mobile nuclear proteins away from chromatin. Molecular Biology of the Cell 27: 4011-4020 (2016). [pdf]

C.R. Pfeifer, C. Alvey, J. Irianto, and D.E. Discher.

Genome variation across cancers scales with tissue stiffness – an invasion-mutation mechanism and implications for immune cell infiltration.

Current Opinion in Systems Biology 2: 102-113 (2017). [pdf]

D.E. Discher, L. Smith, S. Cho, M. Colasurdo, A.J. García, S.A. Safran.

Matrix Mechanosensing: from scaling concepts in ‘omics data to mechanisms in the nucleus, cancer, and regeneration.

Annual Review of Biophysics 46: 295-315 (2017). [pdf]

S. Cho, J. Irianto, and D.E. Discher.

Mechanosensing by the nucleus: from pathways to scaling relationships.

Journal of Cell Biology 216: 305-315 (2017). [pdf]

A. Buxboima, J. Irianto, J. Swift, A. Athirasalaa, J-W. Shin, F. Rehfeldt, and D.E. Discher

Coordinated increase of nuclear tension and lamin-A with matrix stiffness outcompetes
lamin-B receptor that favors soft tissue phenotypes.

Molecular Biology of the Cell 28: 3333-3348 (2017). [pdf]

I.L. Ivanovska, J. Swift, K. Spinler, P.C.D.P. Dingal, S. Cho, and D.E. Discher.

Cross-linked matrix rigidity and soluble retinoids synergize in nuclear lamina regulation of stem cell differentiation.

Molecular Biology of the Cell 28: 2010-2022 (2017). [pdf]

E.A. Novikova, M. Raab, D.E. Discher, and C. Storm.

Persistence-driven durotaxis: Generic, directed motility in rigidity gradients.

Physical Review Letters 118: 078103 (2017). [pdf]

M. Raab and D.E. Discher.

Matrix rigidity regulates microtubule network polarization in migration.

Cytoskeleton 74:114-124 (2017). [pdf]

J. Irianto, C. Pfeifer, Y. Xia, D.E. Discher.

Snapshot: mechanosensing matrix.

Cell 165: 1820 (2016). [pdf]

K.K. Chiou, J.W. Rocks, C. Yingxian Chen, S. Cho, K.E. Merkus, A. Rajaratnam, P. Robison, M. Tewari, K. Vogel, S.F. Majkut, B.L. Prosser, D.E. Discher, and A.J. Liu.

Mechanical signaling coordinates the embryonic heartbeat.

PNAS 113: 8939-44 (2016). [pdf]

P.C.D.P. Dingal, A.M. Bradshaw, S. Cho,  M. Raab, A. Buxboim, J. Swift, and D.E. Discher. Fractal heterogeneity in minimal matrix models of scars modulates stiff-niche stem-cell responses via nuclear exit of a mechanorepressor.

Nature Materials 14: 951–960 (2015). [pdf]  +  press release

J-W. Shin, and D.E. Discher.Blood and immune cell engineering: cytoskeletal contractility and nuclear rheology impacts cell lineage and localization.

Bioessays 37:633-42 (2015) [pdf] 

I.L. Ivanovska, J-W. Shin, J. Swift, and D.E. Discher.

Stem cell mechanobiology – diverse lessons from bone marrow.

Trends in Cell Biology 25:523-32 (2015).  [pdf] 

J-W. Shin, A. Buxboim, K.R. Spinler, J. Swift, D.A. Christian, C.A. Hunter, C. Léon, C. Gachet, P.C. Dave P. Dingal, I.L. Ivanovska, F. Rehfeldt, J.A. Chasis, and D.E. Discher. Contractile forces sustain and polarize hematopoiesis from stem and progenitor cells.

Cell Stem Cell 14: 81-93 (2014).  ** Cover Article ** [pdf]  +  press release

T. Harada, J. Swift, J. Irianto, J-W. Shin, K.R. Spinler, A. Athirasala, R. Diegmiller, P.C. D.P. Dingal, I.L. Ivanovska, and D.E. Discher.

Nuclear lamin stiffness is a barrier to 3D-migration, but softness can limit survival.

Journal of Cell Biology 204:669-682 (2014). [pdf]  +  press release

P.C.D.P. Dingal, and D.E. Discher.

Systems Mechano-Biology: Tension-inhibited Protein Turnover is sufficient to physically control Gene Circuits. Biophysical Journal Special Issue on Quantitative Cell Biology

107: 2734-2743 (2014). [pdf] 

A. Buxboim, J. Swift, J. Irianto, K. Spinler, P.C. D.P. Dingal, A. Athirasala, Y-R.C. Kao, S. Cho, T. Harada, J-W. Shin, and D.E.DischerMatrix elasticity regulates lamin-A,C phosphorylation and turnover with feedback to actomyosin.

Current Biology 24: 1909-17 (2014). [pdf] 

J. Swift, I.L. Ivanovska, A. Buxboim, T. Harada, P.C. D.P. Dingal, J. Pinter, J.D. Pajerowski, K. Spinler, J-W. Shin, M. Tewari, F. Rehfeldt, D.W. Speicher, and D.E. Discher

Nuclear Lamin-A Scales with Tissue Stiffness and Enhances Matrix-directed Differentiation.

Science 341: 1240104-1 to 15 (2013). [pdf]   + Commentary [pdf]  +  press

S. Majkut, T. Idema, J. Swift, C. Krieger, A. Liu, and D.E. DischerHeart-specific stiffening in early embryos parallels matrix and myosin levels to optimize beating.

Current Biology (online 2013). [pdf]  +  press release

J-W. Shin, K.R. Spinler, J. Swift, J. Chasis, N. Mohandas, D.E. Discher.

Lamins regulate cell trafficking and lineage maturation of adult human hematopoietic cells.

PNAS 110: 18892–18897 (2013). [pdf]

J. Swift, T. Harada, A. Buxboim, J-W Shin, H-Y Tang, D.W. Speicher and D.E. Discher. Label-free mass spectrometry exploits dozens of detected peptides to quantify lamins in wildtype and knockdown cells. Nucleus10.4161/nucl.27413 (2013).

M. Raab, J. Swift, P.C.D.P. Dingal, P. Shah, J-W. Shin, and D.E. Discher. Crawling from Soft to Stiff Matrix polarizes the Cytoskeleton and phospho-regulates Myosin-II heavy chain. Journal of Cell Biology 199: 669-683 (2012). [pdf]

F.Rehfeldt, A.E.X. Brown, M. Raab, S. Cai, A.L. Zajac, A. Zemel, and D.E. Discher.  Hyaluronic Acid Matrices of Tunable Elasticity in 2D and 3D show Matrix Stiffness Dictates Cytoskeletal Order and Myosin-II phosphorylation within Stem Cells.

Integrative Biology 4: 422-30 (2012). [pdf]

J-W. Shin, J. Swift, K.R. Spinler, and D.E. Discher. Myosin-II inhibition and soft 2D matrix maximize multi-nucleation and cellular projections typical of platelet-producing megakaryocytes PNAS 108: 11458-11463 (2011). [pdf]

A. Buxboim, I. Ivanova, and D.E. Discher. Matrix Elasticity, Cytoskeletal Forces, and Physics of the Nucleus:  how deeply do cells ‘feel’ outside and in?

Journal of Cell Science123: 297-308 (2010). 

D.E. Discher, D.J. Mooney, P.W. Zandstra.

Growth factors, matrices, and forces combine and control stem cells.

Science 324:1673-1677 (2009) [pdf]

A. Engler, C. Carag, C. Johnson, M. Raab, H-Y. Tang, D. Speicher, J. Sanger, J. Sanger, and D.E. Discher. Embryonic cardiomyocytes beat best on a matrix with heart-like elasticity:  scar-like rigidity inhibits beating.

Journal of Cell Science 121: 3794-3802 (2008).  ** Editor's Highlight. **

J.D. Pajerowski, K.N. Dahl, F.L. Zhong, P.J. Sammak, and D.E. Discher.

Physical plasticity of the nucleus in stem cell differentiation.

PNAS 104:15619-15624 (2007).[pdf]

A. Engler, S. Sen, H.L. Sweeney, D.E. Discher.

Matrix elasticity directs stem cell lineage specification.

Cell 126:677-689 (2006). [pdf]

D.E. Discher, P. Janmey, Y-L. Wang.

Tissue cells feel and respond to the stiffness of their substrate.

Science 310:1139-1143 (2005). [pdf]

A. Engler, L. Bacakova, C. Newman, A. Hategan, M. Griffin, and D.E. Discher.

Substrate compliance versus ligand density in cell on gel responses.

Biophysical Journal 86:617-628 (2004). [pdf]

A. Engler, M. Griffin, S. Sen, C. Bonnemann, H.L. Sweeney, and D.E. Discher.

Myotubes Differentiate Optimally on Substrates with Tissue-like Stiffness: Pathological Implications of Stiff or Soft Microenvironments.

Journal of Cell Biology 166:877-887 (2004).[pdf]

some 'Marker of Self' Immunobiology and Cell Membranes

C.M. Alvey, K.R. Spinler, J. Irianto, C.R. Pfeifer, B. Hayes, Y. Xia, S. Cho, P.C.P.D. Dingal, J. Hsu, L. Smith, M. Tewari, and D.E. Discher. SIRPA-inhibited, marrow-derived macrophages engorge, accumulate, and differentiate in antibody-targeted regression of solid tumors. Current Biology 27: ### (2017). doi: 10.1016/j.cub.2017.06.005. [pdf] press

C. Alvey and D.E. Discher. Engineering macrophages to eat cancer: from ‘Marker of Self’ CD47 and phagocytosis to differentiation.

Journal of Leukocyte Biology 102: 31-40 (2017). [pdf] 

N.G. Sosale, I.I. Ivanovska, R.K. Tsai, J. Swift, P.Z. Zoltick, and D.E. Discher.

‘Marker of Self’ CD47 on lentiviral vectors decreases macrophage-mediated clearance and increases delivery to SIRPA-expressing lung carcinoma tumors. 

Molecular Therapy — Methods & Clinical Development (2016) 3: 16080. [pdf] 

N.G. Sosale, T. Rouhiparkouhi, A.M. Bradshaw, R. Dimova, R. Lipowsky, and D.E. Discher. Cell Rigidity and Shape override CD47’s ‘Self’ signaling in Phagocytosis by Hyperactivating Myosin-II.   Blood 125:542-52 (2015). [pdf]

K.R. Spinler, J-W. Shin, M.P. Lambert, D.E. Discher. Myosin-II repression favors pre/pro-platelets, but shear-activation generates platelets and fails in macrothrombocytopenia. Blood 125:525-33 (2015). [pdf]

N.G. Sosale, K.R. Spinler, C. Alvey, and D.E. DischerMacrophage engulfment of a cell or nanoparticle is regulated by  unavoidable opsonization, a species-specific ‘Marker of Self’ CD47, and target physical properties.

Current Opinion in Immunology 35:107-12 (2015). [pdf]

P.L. Rodriguez, T. Harada, D.A. Christian, D.A. Pantano, R.K. Tsai, and D.E. Discher. Minimal 'Self' peptides that inhibit phagocytic clearance and enhance delivery of nanoparticlesScience 339: 971-975 (2013). [pdf]  +  press

R. Tsai, P. Rodriguez, and D.E. Discher.  Self inhibition of phagocytosis:  the affinity of ‘Marker of Self’ CD47 for SIRPα dictates potency but only at low expression levels.

Blood Cells, Molecules, and Disease 45: 6774 (2010).

R.K. Tsai and D.E. Discher. Inhibition of "self" engulfment through deactivation of myosin-II at the phagocytic synapse between human cells.

Journal of Cell Biology 180:989-1003 (2008). [pdf] **Cover Article**

S. Subramanian, E. Boder, and D.E. Discher. Phylogenetic divergence of CD47 Interactions with human Signal Regulatory Protein-a reveals locus of species specificity.

Journal of Biological Chemistry 282:1805-18 (2007).

S. Subramanian, R. Parthasarthy, E. Boder, and D.E. Discher.

Species- and cell-specific adhesive interactions between CD47 and human SIRPa.

Blood 107:2548-2556 (2006). [pdf]

S. Subramanian, R. Tsai, S. Sen, K.N. Dahl, and D.E. Discher.

Membrane mobility and clustering of Integrin Associated Protein (IAP, CD47) – Major differences between mouse and man and implications for signaling.

Blood Cells, Molecules, and Disease 36: 364-72 (2006).

S. Subramanian, R. Tsai, and D.E. Discher. The ‘Metabolon’, CD47, and the ‘Phagocytic Synapse’: co-localization studies and species divergence. Transfusion Clinique et Biologique Special Issue on Rh Family Proteins 13: 31-38 (2006).

S. Sen, S. Subramanian, and D.E. Discher.

Indentation and adhesive probing of a cell membrane with AFM: model and experiments.

Biophysical Journal 89:3203-3213 (2005). [pdf]

K.N. Dahl, R. Parthasarthy, C. Westhoff, and D.E. Discher. Protein 4.2 is critical to CD47-membrane skeleton attachment in human red cells. Blood 103: 1131-1136 (2004).

K.N. Dahl, C. Westhoff, and D.E. DischerFractional attachment of CD47 (IAP) to the erythrocyte cytoskeleton and visual co-localization with Rh protein complexes.

Blood 101: 1194-1199 (2003).

D.E. Discher.  New insights into red cell membrane organization and micro-elasticity. Current Opinion in Hematology 7: 117-122 (2000).

S.K. Boey, D.H. Boal, and D.E. Discher. Simulations of the erythrocyte cytoskeleton at large deformation I:  microscopic models.  Biophysical Journal 75: 1573-1583 (1998).

D.E. Discher, R. Winardi, P.O. Schischmanoff, M. Parra, J.G. Conboy, and N. Mohandas. Mechanochemistry of protein 4.1's spectrin-actin binding domain: Ternary complex interactions, membrane binding, network integration, structural strengthening.

Journal of Cell Biology130:897-907 (1995).

D.E. Discher, N. Mohandas, E.A. Evans. Molecular maps of red cell deformation: Hidden elasticity and in situ connectivity. Science 266:1032-1035 (1994).

some Materials Biology & Molecular Mechanics

S.F. Majkut and D.E. Discher. Cardiomyocytes from late embryos and neonates do optimal work and striate best on substrates with tissue-level elasticity - Metrics and mathematics. Biomechanics and Modeling in Mechanobiology 11:1219–1225 (2012). [pdf]

C.C. Krieger, X. An, H-Y. Tang, N. Mohandas, D.W. Speicher, &  D.E. Discher. Cysteine Shotgun Mass Spectrometry (CS-MS) reveals dynamic sequence of protein structure changes within mutant and stressed cells. PNAS 108: 8269–8274 (2011). [pdf]

C. Carag, N. Bhasin, M. Tewari, A.E.X. Brown, D. Safer, H.L. Sweeney, and D.E. Discher.  Exon-skipped dystrophins for treatment of Duchenne muscular dystrophy:  Mass Spectrometry mapping of most Exons and Cooperative Domain Designs based on Single Molecule Mechanics.  Cytoskeleton 67:796–807 (2010). [pdf]

A. Buxboim, K. Rajagopal, A.E.X. Brown, and D.E. Discher.

How deeply cells feel:  Methods for thin gels.

Journal of Physics: Condensed Matter 22: 194116 (2010).

A.E.X. Brown, R.I. Litvinov, D.E. Discher, P.K. Purohit, J.W. Weisel. Multiscale Mechanics of Fibrin Polymer: Gel Stretching with Protein Unfolding and Loss of Water.

Science 325:741-744 (2009).[pdf]

S. Sen, A. Engler, and D.E. DischerMatrix strains induced by cells: computing how far cells can feel.  Cell and Molecular Bioengineering 2: 39-48 (2009).  ** Cover Article **

A.E. Brown and D.E. Discher. Conformational changes and signaling in cell and matrix physics. Current Biology19: R781–R789 (2009).

C.P. Johnson, H-Y. Tang, C. Carag, D.W. Speicher, and D.E. Discher.

Forced Unfolding of Proteins Within Cells.

Science 317:663-666 (2007).[pdf]

P. Dalhaimer, D.E. Discher, and T.C. Lubensky. Crosslinked actin networks show liquid crystal elastomer behaviour, including soft-mode elasticity.

Nature Physics 96:354-360 (2007). [pdf]

S. Sen, S. Subramanian, and D.E. Discher.

Indentation and adhesive probing of a cell membrane with AFM: model and experiments.

Biophysical Journal 89:3203-3213 (2005). [pdf]

N. Bhasin, R. Law, G. Liao, D. Safer, J. Elmer, B.M. Discher, H.L. Sweeney, and D.E. Discher. Molecular extensibility of mini-dystrophins and a dystrophin rod construct.

Journal of Molecular Biology 352:795-806 (2005). [pdf]

V. Ortiz, S. Nielsen, M.L. Klein, and D.E. Discher.

Unfolding a linker between helical repeats.

Journal of Molecular Biology 349:638-647 (2005). [pdf]

A. Engler, L. Bacakova, C. Newman, A.Popescu, M. Sheehan, and D.E. Discher. Substrate compliance versus ligand density in cell on gel responses.

Biophysical Journal 86:617-628 (2004).[pdf]

R. Law, P. Carl, S. Harper, P. Dalhaimer, D.W. Speicher, and D.E. Discher.  Cooperativity in forced unfolding of tandem spectrin repeats. Biophysical Journal 84: 533-544 (2003).

R. Law, G. Liao, S. Harper, G. Yang, D.W. Speicher, and D.E. Discher.  Pathway shifts and thermal softening in temperature-coupled forced unfolding of spectrin domains.

Biophysical Journal 85: 3286-3293 (2003).

P. Carl, C. Kwok, G. Manderson, D.W. Speicher, and D.E. Discher.  Forced unfolding modulated by disulfide bonds in the immunoglobulin domains of a cell adhesion molecule.  PNAS 98: 1565-1570 (2001).

some Assemblies -- Polymersomes, Filomicelles, & Molecular Dynamics

A. Mahmud, T. Harada, K. Rajagopal, D.A. Christian, P. Nair, R.Murphy and D.E. Discher. Spray stability of self-assembled filaments for delivery.

Journal of Controlled Release (2017). doi: 10.1016/j.jconrel.2017.05.026  [pdf]

P.R. Nair, K. S. Anbazhagan, K.R. Spinler, M.R. Vakili A. Lavasanifar, and D.E. Discher. Filomicelles from aromatic di-block copolymers increase paclitaxel-induced tumor cell death and aneuploidy compared to aliphatic copolymer.

Nanomedicine 11(12):1551-69 (2016). [pdf]

N. Sancho Oltra, P. Nair, and D.E. Discher. From stealthy polymersomes and filomicelles to “Self” peptide-nanoparticles for cancer therapy.

Annual Reviews of Chemical and Biomolecular Engineering 5:281-299 (2014). [pdf]

K. Spinler, A. Tian,  D.A. Christian, D.A. Pantano, T. Baumgart, and D.E. Discher. Dynamic domains in polymersomes:  mixtures of polyanionic and neutral diblocks respond more rapidly to changes in calcium than pH.  Langmuir 29: 7499-7508 (2013). [pdf]

N. Sancho Oltra, J. Swift, A. Mahmud, K. Rajagopal, S.M. Loverde and D.E. Discher.  Filomicelles in nanomedicine – from flexible, fragmentable, and ligand-targetable drug carrier designs to combination therapy for brain tumors. Journal of Materials Chemistry B – Themed Issue of Nanoparticles in Biology  1: 5177-5185 (2013).

S.M. Loverde, M.L. Klein, D.E. Discher. Improved delivery of the anti-cancer drug Taxol by worm-like PEG-PCL micelles simulated by rational coarse grain molecular dynamics (rCG-MD). Advanced Materials 24: 3823-30 (2012). [pdf]

D. Pantano, P.B. Moore, M.L. Klein, D.E. Discher. Raft registration across bilayers in a molecularly detailed model. Soft Matter 2011, 7, 8182-8191 (2011). [pdf]

D.A. Christian, A. Tian, W.G. Ellenbroek, I. Leventhal, K. Rajagopal, P.A. Janmey, A.J. Liu, T. Baumgart, D.E. Discher. Spotted vesicles, striped micelles and Janus assemblies induced by ligand binding. Nature Materials 8:843-849 (2009). [pdf]

D.A. Christian, S. Cai, O. Garbuzenko, T. Harada, A. Zajac, T. Minko, and  D.E. Discher. Flexible filaments for in vivo imaging and delivery:  Persistent circulation of filomicelles opens the dosage window for sustained tumor shrinkage. 

ACS - Molecular Pharmaceutics 6: 1343–1352 (2009).

Y. Kim, M. Tewari, D. Pajerowski, S. Cai, S. Sen, W. Jason, S. Sirsi, G. Lutz, D.E. Discher. Polymersome delivery of RNAi and antisense oligonucleotides. Journal of Controlled Release134: 132–140 (2009). ** Cover Article and Editor's Highlight.  **

Y. Geng, P. Dalhaimer, S. Cai, R. Tsai, M. Tewari, T. Minko, and D.E. Discher.

Shape effects of filaments versus spherical particles in flow and drug delivery.

Nature Nanotechnology 2:249-255 (2007). [pdf]

F. Ahmed, R. Pakunlu, G. Srinivas, A. Brannon, F.S. Bates, M.L. Klein, T. Minko, and D.E. Discher. Shrinkage of a rapidly growing tumor by drug-loaded Polymersomes: pH-Triggered release through copolymer degradation.

ACS - Molecular Pharmaceutics 3:340-350 (2006). [pdf]

F. Ahmed and D.E. Discher. Controlled release from polymersome vesicles blended with PEO-PLA or related hydrolysable copolymer.

Journal of Controlled Release 96:37-53 (2004). [pdf]

** Best Paper 2004: Jorge Heller Award **

D.E. Discher and A. Eisenberg.

Polymer Vesicles.

Science 297:967-973 (2002). [pdf]