Welcome to BioLines Laboratory

Publications

54.    S.E. Park, A. Georgescu, D. Huh, “Organoids-on-a-chip,” Science 2019, Read Here

53.    J. Seo, W.Y. Byun, F. Alisafaei, A. Georgescu, Y.S. Yi, M. Massaro-Giordano, V.B. Shenoy, V. Lee, V. Bunya, D. Huh, “Multiscale reverse engineering of the human ocular surface,” Nat. Med. 2019, Read Here

52.    J. Paek, S.E. Park, Q. Lu, K.T. Park, M. Cho, J.M. Oh, K.W. Kwon, Y.S. Yi, J.W. Song, H.I. Edelstein, J. Ishibashi, W. Yang, J.W. Myerson, R.Y. Kiseleva, P. Aprelev, E.D. Hood, D. Stambolian, P. Seale, V.R. Muzykantov, D. Huh, “Microphysiological engineering self-assembled and perfusable microvascular beds for the production of vascularized three-dimensional human microtissues,” ACS Nano 2019, Read Here

51.    S.E. Park, A. Georgescu, J.M. Oh, K.W. Kwon, D. Huh, “Polydopamine-based interfacial engineering of three-dimensional cell culture for production and long-term maintenance of in vitro tissue constructs,” ACS Appl. Mater. Interfaces 2019, Read Here

50.    S.E. Park, J. Ahn, H-.E. Jeong, K-.I. Song, I. Youn, D. Huh*, S. Chung*, “In vitro model of threedimensional peripheral myelination,” submitted. *co-corresponding authors

49.    J.Y. Park, C. Blundell, Y.S. Yi, J. Jang, J.H. Park, M. Ahn, D.W. Cho, D. Huh, “Multimodal engineering of functional living tissues with autonomous mechanical activity using naturally derived cell-instructive hydrogels,” submitted.

48.    M. Mondrinos, Y.S. Yi, C. Blundell, J. Seo, M. Osborn, H. Ahmadzaeh, I. Lee, T. Chun, S.M. Kim, V.B. Shenoy, D. Huh, “Surface-directed cellular sculpting of engineered tissue anisotropy,” submitted.

47.    J. Chang, J. Lee, A. Georgescu, D. Huh, T. Kang, “Large-scale production of nanoparticle arrays on arbitrary surfaces,” Nano Lett. 2019. Read Here

46.    P. Elkington, M. Lerm, N. Kapoor, R. Mahon, E. Pienaar, D. Huh, D. Kaushal, D. Kaushal, L. Schlesinger, “In vitro granuloma models of tuberculosis: potential and challenges,” J. Infect. Dis. 2019, in press.

45.    J. Seo, D. Huh, “Microphysiological models of human organs: a case study on microengineered lung on-a-chip systems,” Microfluidic Cell Culture Systems, Ed. Borenstein, J. 2019, in press.

44.    D. Huh, “Microphysiological models of the respiratory system,” Biofabrication and 3D Tissue Modeling, Biomaterials Science Series. Ed. Cho, D.-W. 2019, in press.

43.    K. Gkatzis, S. Taghizadeh, D. Huh, D.Y.R. Stainier, S. Bellusci, “Using 3D organoids and lung-on-a-chip methods to study lung development, regeneration, and disease,” Eur. Respir. J. 2018. Read Here

42.    J. Song, J. Paek, K.T. Park, J. Seo, D. Huh. A bioinspired microfluidic model of mucus plug-induced airway injury, Biomicrofluidics 2018, (Editor’s pick). Read Here

41.    A.J. Clippinger, D. Allen, A.M. Jarabek, M. Corvaro, M. Gaça, S. Gehen, J.A. Hotchkiss, G. Patlewicz, J. Melbourne, P. Hinderliter, M. Yoon, D. Huh, A. Lowit, B. Buckley, M. Bartels, K. BéruBé, D.M. Wilson, I. Indans, M. Vinken, “Alternative approaches for acute inhalation toxicity testing to address global regulatory and non-regulatory data requirements: An international workshop report,” Toxicol. In Vitro 2018 48, 53-70. Read Here

40.    C. Blundell, Y.S. Yi, L. Ma, E. Tess, M. Farrell, A. Georgescu, L. Aleksunes, D. Huh, “Placental drug transport-ona-chip: a microengineered in vitro model of transporter-mediated drug efflux in the human placental barrier,” Adv. Healthc. Mater. 2017 (cover article). Read Here

39.    M. Mondrinos, Y.S. Yi, N.K. Wu, X. Ding, D. Huh, “Natural extracellular matrix-derived semipermeable, optically transparent semipermeable membrane inserts for microfluidic cell culture,” Lab Chip 2017, 17, 3146-3158. Read Here

38.    J. Seo, D. Conegliano, M. Farrell, M. Cho, X. Ding, T. Seykora, D.Y. Qing, N.S. Mangalmurti, D. Huh, “A microfluidic model of red blood cell transfusion-induced pulmonary vascular injury,” Sci. Rep. 2017. Read Here

37.    S.H. Yang, J.W. Choi, D. Huh, H.A. Jo, S. Kim, C.S. Lim, J.C. Lee, H.C. Kim, H.M. Kwon, C. Kwak, K.W. Joo, Y.S. Kim, D.K. Kim, “Roles of fluid shear stress and retinoic acid on the differentiation of primary cultured human podocytes,” Exp. Cell Res. 2017. Read Here

36.    C. Blundell, E. Tess, A. Schanzer, C. Coutifaris, E. Su, S. Parry, D. Huh, “A microengineered model of the human placental barrier,” Lab Chip 2016. Read Here

35.    S. Han, Y. Shin, H.E. Jeong, J. Jeon, R. Kamm, D. Huh, L. Sohn, and S. Chung, “Constructive remodeling of a synthetic endothelial extracellular matrix,” Sci. Rep. 2016. Read Here

34.    Y.S. Choi, E. Hyun, J. Seo, C. Blundell, H.C. Kim, E. Lee, S. Lee, W.K. Moon, D. Huh, “A microengineered model of early-stage breast cancer,” Lab Chip 2015. Read Here

33.    J.S. Lee, Y.M. Han, H.C. Kim, C.J. Kim, R. Romero, J.S. Hong, D. Huh, “Placenta-on-a-chip: a novel platform to study the biology of human placenta,” J. Matern. Fetal Neonatal Med. 2015. Read Here

32.    D. Huh, “A human breathing lung-on-a-chip,” Ann. Am. Thorac. Soc. 2015. Read Here

31.    E. Esch, A. Bahinski, D. Huh, “Organs-on-chips at the frontiers of drug discovery,” Nat. Rev. Drug Discov. 2014. Read Here

30.    D.Y. Qing, D. Conegliano, J. Seo, J.P. Reilly, M.G. Shashaty, G.S. Worthen, D. Huh, N.J. Meyer, N.S. Mangalmurti, “RBC transfusion increases susceptibility to lung inflammation through high mobility group box 1 released by necroptosis of lung endothelial cells,” Am. J. Respir. Crit. Care Med. 2014. Read Here

29.    S. Choi, Y. Hong, I. Lee, D. Huh, T.-J. Jeon, S.M. Kim, “Effects of various extracellular matrix proteins on the growth of HL-1 cardiomyocytes,” Cells Tissues Organs 2014. Read Here

28.    D. Huh*, H.J. Kim, J.P. Fraser, D.E. Shea, M. Khan, A. Bahinski, G.A. Hamilton, D.E. Ingber*, “Microfabrication of human organs-on-chips,” Nat. Protoc. 2013. *co-corresponding authors Read Here

27.    D. Huh, D.C. Leslie, B.D. Matthews, J.P. Fraser, S. Jurek, G.A. Hamilton, K.S. Thorneloe, M.A. McAlexander, D.E. Ingber, “A human disease model of drug toxicity-induced pulmonary edema in a lung-on-a-chip microdevice,” Science Trans. Med. 2012. (cover article); Awarded the NC3Rs Annual 3Rs Prize; Featured in Science, Reuters, MIT-Tech. Review, Harvard Gazette, IEEE Spectrum, Fox News, Chosun Ilbo, Kookmin Ilbo, KBS News, etc.

26.    J. Park, K.B. Kim, J. Lee, H.C. Kim, D. Huh*, “Organomimetic Microsystems Technologies,” Biomed. Eng. Lett. 2012.; Awarded KSMBE Best Publication Award Read Here

25.    D. Huh, Y. Torisawa, G.A. Hamilton, H.J. Kim, D.E. Ingber, “Microengineered physiological biomimicry: organs-onchips,” Lab Chip 2012. Read Here

24.    H.J. Kim, D. Huh, G.A. Hamilton, D.E. Ingber, “Human gut-on-a-chip inhabited by microbial flora that experiences intestinal peristalsis-like motions and flow,” Lab Chip 2012. Read Here

23.    D. Huh, G.A. Hamilton, D.E. Ingber, “From three dimensional cell culture to organs-on-chips,” Trends Cell Biol. 2011. Read Here

22.    T. Mammoto, A. Mammoto, T. Tat, Y. Torisawa, A. Gibbs, R. Derda, R. Mannix, M. de Bruijin, C.W. Yung, D. Huh, D.E. Ingber, “Mechanochemical control of mesenchymal condensation and embryonic tooth organ formation,” Dev. Cell 2011. Read Here

21.    D. Huh, B. D. Matthews, A. Mammoto, M. Montoya, H.Y. Hsin, D.E. Ingber, “Reconstituting organ-level lung functions on a chip,” Science 2010, 328, 1662-1668 Read Here; Named to Top 100 Science Stories of 2010 (Discover Magazine); Faculty of 1000 Exceptional; Featured in Nature, Lab on a Chip, Scientific American, Popular Mechanics, Boston Globe, ABC,CBS, Bloomberg, LA times , etc.

20.    K. L. Mills, D. Huh, S. Takayama, M. D. Thouless, “Instantaneous fabrication of arrays of normally closed, adjustable, and reversible nanochannels by tunnel cracking,” Lab Chip 2010. Read Here

19.    H. Tavana, C.-H. Kuo, Q.Y. Lee, B. Mosadegh, D. Huh, P.J. Christensen, J.B. Grotberg, S. Takayama, “Dynamics of Liquid Plugs of Buffer and Surfactant Solutions in a Micro-Engineered Pulmonary Airway Model,” Langmuir 2010. Read Here

18.    A. Mammoto, K.M. Connor, T. Mammoto, C.W. Yung, D. Huh, C.M. Aderman, G. Mostoslavsky, L.E.H. Smith, D.E. Ingber, “A Mechanosensitive Transcriptional Mechanism that Controls Angiogenesis,” Nature 2009. Read Here

17.    D. Huh, C.H. Kuo, J.B. Grotberg, S. Takayama, “Gas-Liquid Two-Phase Flow Patterns in Rectangular Polymeric Microchannels”, New J. Phys. 2009. Read Here

16.    Y. Zheng, H. Fujioka, S. Bian, Y. Torisawa, D. Huh, S. Takayama, J.B. Grotberg, “Liquid Plug Propagation in Flexible Microchannels – A Small Airway Model.” Phys. Fluids 2009.Read Here

15.    H. Tavana, D. Huh, J.B. Grotberg, S. Takayama, “Microfluidics, Lung Surfactant, and Respiratory Disorders,” Labmedicine 2009. Read Here

14.    H. Tavana, D. Huh, J.B. Grotberg, S. Takayama, “Pulmonary Airways on a Chip: A New Approach to Study Respiratory Disorders,” BioForum Europe 2009.

13.    Y. Kamotani, T. Bersano-Begey, N. Kato, Y.-C. Tung, D. Huh, J.W. Song, S. Takayama, “Individually Programmable Cell Stretching Microwell Arrays Actuated by a Braille Display,” Biomaterials 2008. Read Here

12.    N. Douville, D. Huh, S. Takayama, “DNA Linearization through Confinement in Nanofluidic Channels,” Anal. Bioanal. Chem. 2008. Read Here

11.    D. Huh, K. L. Mills, X. Zhu, M.A. Burns, M. D. Thouless, S. Takayama, “Tuneable Elastomeric Nanochannels for Nanofluidic Manipulation,” Nat. Mater. 2007. Read Here

10.    D. Huh, H. Fujioka, Y.-C. Tung, N. Futai, R. Paine III, J.B. Grotberg, S. Takayama, “Acoustically Detectable Cellular-Level Lung Injury Induced by Fluid Mechanical Stresses in Microfluidic Airway Systems,” Proc. Nat. Acad. Sci. U. S. A. 2007. Read Here; Featured in Nature and Lab on a Chip.

9.    D. Huh, J.H. Bahng, Y. Ling, H.-H. Wei, O.D. Kripfgans, J.B. Fowlkes, J.B. Grotberg, S. Takayama, “Gravity-Driven Microfluidic Particle Sorting Device with Hydrodynamic Separation Amplification,” Anal. Chem. 2007. Read Here

8.    D. Huh, Y. Kamotani, J.B. Grotberg, S. Takayama, “Engineering pulmonary epithelia and their mechanical microenvironments” Micro- and Nanoengineering of the Cell Microenvironment: Technologies and Applications, Ed. Khademhosseini, 2007. Read Here

7.    Y. Torisawa, B. Chueh, D. Huh, P. Ramamurthy, T.M. Roth, K.F. Barald, S. Takayama, “Efficient Formation of Uniform-Sized Embryoid Bodies Using a Compartmentalized Microchannel Device,” Lab Chip 2007. Read Here

6.    B. Chueh, D. Huh, C.R. Kyrtsos, T. Houssin, N. Futai, S. Takayama, “Leakage-Free Bonding of Porous Membranes into Layered Microfluidic Array Systems,” Anal. Chem. 2007. Read Here

5.    A.J. Calderón, Y. Heo, D. Huh, N. Futai, S. Takayama, J.B. Fowlkes, J.L. Bull, “A Microfluidic Model of Bubble Lodging in Microvessel Bifurcations,” Appl. Phys. Lett. 2006. Read Here

4.    D. Huh, W. Gu, Y. Kamotani, J.B. Grotberg, S. Takayama, “Microfluidics for Flow Cytometric Analysis of Cells and Particles,” Physiol. Meas. 2005. Read Here; Best Review published in Physiol. Meas. in 2005.

3.    Y. Kamotani, D. Huh, N. Futai, S. Takayama, “At the Interface: Advanced Microfluidic Assays for Study of Cell Function,” Therapeutic Micro/NanoTechnology, Ed. Bhatia, S.; Desai, T. 2004.

2.    D. Huh, A.H. Tkaczyk, J.H. Bahng, Y Chang, H.-H. Wei, J.B. Grotberg, C.-J. Kim, K. Kurabayashi, S. Takayama, "Reversible Switching of High-Speed Air-Liquid Two-Phase Flows Using Electrowetting-Assisted Flow-Pattern Change," J. Am. Chem. Soc. 2003. Read Here

1.    D. Huh, Y.-C. Tung, H.-H. Wei, J.B. Grotberg, S.J. Skerlos, K. Kurabayashi, S. Takayama, "Use of Air-Liquid TwoPhase Flow in Hydrophobic Microfluidic Channels for Disposable Flow Cytometers," Biomed. Microdev. 2002. Read Here