University of Pennsylvania · Department of Bioengineering


113) Wei, Y., Yan, L., Luo, L., Gui, T., Amirshaghaghi, A., You, T., Tsourkas, A., Qin, L.*, Cheng, Z.*(2020) Phospholipase A2 inhibitor-loaded micellar nanoparticles attenuate inflammation and mitigate osteoarthritis progression. Science Advances. In press. *Contributed equally.

112) Teng, C.W., Amirshaghaghi, A., Cho, S.S., Cai, S.S., Singh, Y., De Ravin, E., Miller, J., Busch, T.M., Cheng, Z., Singhal, S., Delikatny, E., Tsourkas, A., Lee, J.Y.K. (2020) Combined fluorescence-guided surgery and photodynamic therapy for glioblastoma multiforme using cyanine and chlorin nanocluster. Journal of Neuro-Oncology, in press.

111) Higbee-Dempsey, E., Amirshaghaghi, A., Matthew, C., Bouché, M., Kim, J., Cormode, D., Tsourkas, A. (2020) Biodegradable gold nanoclusters with improved excretion due to pH-triggered hydrophobic-to-hydrophilic transition. Journal of the American Chemical Society, 142(17), 7783-7794. PMCID:PMC7238296.

110) Wei, Y., Luo, L., Gui, T., Yu, F., Yan, L., Yao, L., Zhong, L., Yu, W., Han, B., Patel, J., Beier, F., Shao, Z., Han, L., Mauck, R.L., Tsourkas, A., Ahn, J., Cheng, Z.*, Qin, L.* (2020) Targeting cartilage EGFR pathway for osteoarthritis treatment. Science Translational Medicine, In press. *Contributed equally.

109) Kartha, S., Yan, L., Ita, M.E., Amirshaghaghi, A., Luo, L., Wei, Y., Tsourkas, A., Winkelstein, B.A., Cheng, Z. (2020) Phospholipase A2 inhibitor-loaded phospholipid micelles abolish neuropathic pain. ACS Nano, 14(7), 8103-8115. PMCID:PMC7438274.

108) Luo, L., Yan, L., Amirshaghaghi, A., Wei, Y., You, T., Singhal, S., Tsourkas, A.*, Cheng, Z.* (2020) Indocyanine green (ICG)-coated polycaprolactone (PCL) micelles for fluorescence imaging of tumors. ACS Applied Bio Materials, 3(4), 2344-2349.*Contributed equally. PMCID:PMC7245056.

107) Moon, B.F., Iyer, S.K., Hwuang, E., Solomon, M.P., Hall, A.T., Kumar, R., Josselyn, N.J., Higbee-Dempsey, E.M., Tsourkas, A., Imai, A., Okamoto, K., Saito, Y., Pilla, J.J., Gorman III, J.H., Gorman, R.C., Tschabrunn, C., Keeney, S.J., Castillero, E., Ferrari, G., Jockusch, S., Shou, H., Ferrari, V.A., Han, Y., Gulhane, A., Litt, H., Matthai, W., Witschey, W.R. (2020) Iron imaging in myocardial infarction reperfusion injury. Nature Communications, 11(1), 3273. PMCID:PMC7324567.

106) Cho, S., Sheikh, S., Teng, C., Georges, J., Yang, A., De Ravin, E., Buch, L., Li, C., Appelt, D., Delikatny, E., Petersson, E., Tsourkas, A., Dorsey, J., Singhal, S., Lee, J. (2020) Evaluation of diagnostic accuracy following the coadministration of delta-aminolevulinic-acid and second window indocyanine green in rodent and human glioblastomas. Molecular Imaging and Biology, 22(5), 1266-1279.

105) Minutolo, N.G., Sharma, P., Poussin, M., Shaw, L., Brown, D., Hollander, E., Smole, A., Rodriguez-Garcia, A., Hui, J., Zappala, F., Tsourkas, A., Powell, D.J. (2020) Quantitative control of gene-engineered T cell activity through covalent attachment of targeting ligands to a universal immune receptor. Journal of the American Chemical Society, 142(14), 6554-6568. PMCID:PMC7306176.

104) Liu, J., Lan, Z., Ferrari, C., Stein, J., Higbee-Dempsey, E., Yan, L., Cheng, Z., Issadore, D.*, Tsourkas, A.* (2020) Use of oppositely-polarized external magnets to improve the accumulation and penetration of magnetic nanocarriers into solid tumors. ACS Nano. 14(1), 142-152. PMCID:PMC7002255. (*co-corresponding authors)

103) Yan, L., Luo, L., Amirshaghaghi, A., Miller, J., Meng, C., You, T., Busch, T.M., Tsourkas, A., Cheng, Z. (2019) Dextran-benzoporphyrin derivative (BPD) coated superparamagnetic iron oxide nanoparticle (SPION) micelles for T2-weighted magnetic resonance imaging and photodynamic therapy. Bioconjugate Chemistry, 30(11), 2974-2981. PMCID:PMC7241292.

102) Higbee-Dempsey, E., Amirshaghaghi, A., Case, M.J., Miller, J., Busch, T.M., Tsourkas, A. (2019) Indocyanine green coated gold nanoclusters for photoacoustic imaging and photothermal therapy. Advanced Therapeutics. 2(9), 1900088. PMC in process.

101) Wang, H.H., Tsourkas, A. (2019) Cytosolic delivery of inhibitory antibodies with cationic lipids. Proceedings of the National Academy of Science, 116(44), 22132-22139. PMCID:PMC6825285.

100) Liu, J.F., Jang, B., Issadore, D., Tsourkas, A. (2019) Use of magnetic fields and nanoparticles to trigger drug release and improve tumor targeting. WIREs Nanomedicine and Nanobiotechnology, 11(6), e1571. PMCID:PMC6788948.

99) Bouché, M., Pühringer, M., Amirshaghaghi, A., Tsourkas, A., Teasdale, I., Cormode, D.P. (2019) Activatable hybrid polyphosphazene-AuNP nanoprobe for ROS detection by bimodal PA/CT imaging. ACS Applied Materials and Interfaces, ACS Applied Material Interfaces, 11(32), 28648-28656. PMCID:PMC7039041.

98) Amirshaghaghi, A., Yan, L., Miller, J., Daniel, Y., Stein, J.M., Busch, T.M., Cheng, Z., Tsourkas, A. (2019) Chlorin e6-coated superparamagnetic iron oxide nanoparticle (SPION) nanoclusters as a theranostic agent for dual-mode imaging and photodynamic therapy. Scientific Reports, 9(1), 2613. PMCID:PMC6385362

97) Newton, A.D., Predina, J.D., Corbett, C.J., Frenzyl-Sulyok, L.G., Xia, L., Peterson, E.J., Tsourkas, A., Nie, S., Delikatny, E.J., Singhal, S. (2018) Optimization of second window indocyanine green for intraoperative near-infrared imaging of thoracic malignancies. Journal of the American College of Surgeons, 228(2), 188-197. PMCID:PMC6348120

96) Sheikh, S., Saxena, D., Tan, X., Amirshaghaghi, A., Tsourkas, A., Brem, S., Dorsey, J. (2018) An integrated stress response agent that modulates DR5 dependent TRAIL synergy reduces patient derived glioma stem cell viability. Molecular Cancer Research, 17(5), 1102-1114.

95) Ackerman, D.S., Altun, B., Kolodieznyi, D., Bruchez, M., Tsourkas, A., Jarvik, J.W. (2018) Antibody-linked FAPs for antigen detection and cell ablation. Bioconjugate Chemistry, 30(1), 63-69. PMCID:PMC7067197.

94) Amirshaghaghi, A., Altun, B., Nwe, K., Yan, L., Stein, J.M., Cheng, Z., Tsourkas, A. (2018) Site-specific labeling of cyanine and porphyrin dye-stabilized nanoemulsions with affibodies for cellular targeting. Journal of the American Chemical Society, 140(42), 13550-13553. PMCID:PMC6465177.

93) Liu, J.F., Neel, N., Dang, P., Lamb, M., McKenna, J., Rodgers, L., Litt, B., Cheng, Z., Tsourkas, A.*, Issadore, D.* (2018). Radiofrequency-triggered drug release from nanoliposomes with millimeter-scale resolution using a superimposed static gating field. Submitted. Small, 14(44), e1802563. PMCID:PMC6397654 (*co-corresponding authors).

92) Hajfathalian, M., Amirshaghaghi, A., Naha, P.C., Chhour, P. Hsu, J.C., Douglas, K., Dong, Y., Sehgal, C.M., Tsourkas, A., Neretina, S., Cormode, D.P. (2018) Wulff in a cage gold nanoparticles as contrast agents for computed tomography and photoacoustic imaging. Nanoscale, 10(39), 18749-18757. PMCID:PMC6190607.

91) Khoshnejad, M., Greineder, C.F., Pulsipher, K., Villa, C.H., Altun, B., Pan, D., Tsourkas, A., Dmochowski, I., Muzykantov, V. (2018) Ferritin nanocages with biologically orthogonal conjugation for vascular targeting and imaging. Bioconjugate Chemistry, 29(4), 1209-1218. PMCID:PMC6686135.

90) Kayyali M.N., Wooltorton, J.R.A., Ramsey, A.J., Lin, M., Chao, T.N., Tsourkas, A., O’Malley, B.W., Li, D. (2018) A novel nanoparticle delivery system for targeted therapy of noise-induced hearing loss. Journal of Controlled Release, 279, 243-250. PMCID:PMC6344933.

89) Kayyali, M.N., Ramsey, A.J., Dempsey, E.M.H., Cui, Z., Yan, L., Morstadt, L., O’Malley, B.W., Tsourkas, A., Li, D. (2018) The development of a nano-based approach to alleviate cisplatin-induced ototoxicity. Journal of the Association for Research in Otolaryngology, 19(2), 123-132. PMCID:PMC5878149.

88) Yan, L., Amirshaghaghi, A., Huang, D. Miller, J., Stein, J.M., Busch, T.M., Cheng, Z.*, Tsourkas, A.* (2018) Protoporphyrin IX (PpIX)-coated superparamagnetic iron oxide nanoparticle (SPION) nanoclusers for magnetic resonance imaging and photodynamic therapy. Advanced Functional Materials, 28(16), 1707030. (*Co-corresponding authors) PMCID:PMC5997278

87) Greineder, C.F., Villa, C.H., Walsh, L.R., Kiseleva, R., Hood, E.D., Khoshnejad, M., Warden-Rothman, R., Tsourkas, A., Muzykantov, V.R. (2018). Site-specific modification of single-chain antibody fragments for bioconjugation and vascular immunotargeting. Bioconjugate Chemistry, 29(1), 56-66. PMCID:PMC6477694.

86) Khoshnejad, M., Brenner, J.S., Motley, W., Parhiz, H., Greineder, C., Villa, C.H., Marcos-Contreras, O.A., Tsourkas, A., Muzykantov,V. (2018) Molecular engineering of antibodies for site-specific covalent conjugations using CRISPR/Cas9. Scientific Reports, 8(1), 1760. PMCID:PMC5789018.

85) Habibollahi, P., Hui, J., Noji, M., Tsourkas, A., Nadolski, G., Hunt, S., Gade, T. (2017) Development of a novel bispecific antibody for targeted immunotherapy of hepatocellular carcinoma. Journal of Vascular and Interventional Radiology 2 (28), S57-S58

84) Liu, J., Yadavali, S., Tsourkas, A.*, Issadore, D.* (2017). Microfluidic diafiltration-on-chip using an integrated magnetic peristaltic pump. Lab on a Chip, 17(22), 3796-3803. (*co-corresponding authors) PMCID:PMC6082132.

83) Kartha, S., Yan, L., Weisshaar, C.L., Ita, M.E., Shuvaev, V.V., Tsourkas, A., Winkelstein, B.A., Cheng, Z. (2017) Superoxide dismutase-loaded porous polymersomes as highly efficient antioxidants for treating neuropathic pain. Advanced Healthcare Materials, 6(17). PMCID:PMC5591629.

82) Thawani, J.P., Amirshaghaghi, A., Yan, L., Stein, J., Liu, J., Tsourkas, A. (2017) Photoacoustic-guided surgery with indocyanine green-coated superparamagnetic iron oxide nanoparticle clusters. Small, 13(37). PMCID:PMC5884067.

81) Yan, L., Miller, J., Yuan, M, Liu, J., Higbee, E., Busch, T., Tsourkas, A., Cheng, Z. (2017) Improved photodynamic therapy efficacy of protoporphyrin IX loaded polymeric micelles using erlotinib pretreatment. Biomacromolecules, 18(6), 1836-1844. PMCID:PMC5509032

80) Wang*, H.H., Altun*, B., Nwe, K., Tsourkas, A. (2017) Proximity-based sortase-mediated ligation. Angewandte Chemie, 56(19), 5349-5352. (*contributed equally) PMCID:PMC5537000

79) Li, L., Chao, T., Brant, J., O’Malley, B., Tsourkas, A., Li, D. (2017) Advances in nano-based inner ear delivery systems for the treatment of sensorineural hearing loss. Advanced Drug Delivery Review, 108, 2-12 PMCID:PMC4940320.

78) Sun L., Joh D., Stangl M., Al Zaki A., Murty S., Alonso-Basanta M., Kao G, Tsourkas A., Dorsey J.F. (2016) Theranostic application of mixed gold and iron oxide nanoparticle micelles in glioblastoma multiforme. Journal of Biomedical Nanotechnology, 12(2), 347-356. PMCID:PMC4942305.

77) Nwe, K., Huang, C-H., Qu, F., Warden-Rothman, R., Zhang, C.Y., Mauck, R.L., Tsourkas, A. (2016) Cationic gadolinium chelate for magnetic resonance imaging of cartilaginous defects. Contrast Media and Molecular Imaging, 11(3), 229-235 PMCID:PMC4892988.

76) Karunamuni, R., Al Zaki, A., Popov, A., Delikatny, J., Cormode, D., Tsourkas, A., Maidment, A.D.A. (2016) Development of silica-encapsulated silver nanoparticle as contrast agents in dual-energy mammography. European Radiology, 26(9), 3301-3309. PMCID:PMC4974128.

75) Yan, L., Higbee, E., Liu, R., Tsourkas, A., Cheng, Z. (2016). A simple method for the synthesis of porous polymeric vesicles and their application as MR contrast agent. Journal of Materials Chemistry B, 3(48), 9277-9284. PMCID:PMC4675335.

74) Gao, Q., Yan, L., Chiorazzo, M., Delikatny, E.J., Tsourkas, A., Cheng, Z. (2015) PLA2-responsive and SPIO-loaded phospholipid micelles. Chemical Communications, 51(61), 12313-12315. PMCID:PMC4506235.

73) Yan, L., Crayton, S.H., Thawani, J., Amirshaghaghi, A., Tsourkas, A., Cheng, Z. (2015) A pH-responsive drug delivery platform based on glycol chitosan-coated liposomes. Small, 11(37), 4870-4874. PMCID:PMC4592472.

72) Hui, J.Z., Tamsen, S., Song, Y., Tsourkas, A. (2015) LASIC: Light activated site-specific conjugation of native IgGs. Bioconjugate Chemistry, 26(8), 1456-1460. PMCID:PMC5507662.

71) Giraldo-Vela, J.P., Kang, W., McNaughton, R., Zhang, X., Tsourkas, A., Bao, G., Espinosa, H.D. (2015) Single cell detection of mRNA expression using nanofountain-probe electroporated molecular beacons. Small, 11(20), 2386-2391.

70) Al Zaki, A., Hui, J.Z., Higbee, E., Tsourkas, A. (2015) Biodistribution and clearance of gold-loaded polymeric micelles using 0.9 and 5 nm gold nanoparticles. Journal of Biomedical Nanotechnology, 11(10), 1836-1846. PMCID:PMC4942304.

69) Vargo, K.B., Al Zaki, A., Warden-Rothman, R., Tsourkas, A., Hammer, D.A. (2015) Superparamagnetic iron oxide nanoparticle-loaded micelles stabilized by recombinant oleosin for targeted magnetic resonance imaging. Small, 11(12), 1409-1413.

68) McQuade*, C., Al Zaki*, A., Desai, Y., Vido, M., Sakhuja, T., Cheng, Z., Hickey, R., Joh, D., Park, S-J, Kao, G.D., Dorsey, J.F., Tsourkas, A. (2015).  A multi-functional nanoplatform for imaging, radiotherapy, and the prediction of therapeutic response. Small, 11(7), 834-843 (*contributed equally). PMCID: PMC4329028.

67) Lajud, S.A., Nagda, D.A., Qiao, P., Tanaka, N., Civantos, A., Gu, R., Cheng, Z., Tsourkas, A., O’Malley, B.W., Li, D. (2015) A novel chitosan-hydrogel-based nanoparticle delivery system for local inner ear application. Otology & Neurology, 36(2), 341-341. PMCID:PMC4365436.

66) Murty, S., Gilliland, T., Qiao, P., Tabtieng, T., Higbee, E., Al Zaki, A., Puré, E., Tsourkas, A. (2014) Nanoparticles functionalized with collagenase exhibit improved tumor accumulation in a murine xenograft model. Particle and Particle Systems Characterization, 31, 1307-1312. PMCID:PMC4569138.

65) Eckmann, D.M., Composto, R.J., Tsourkas, A., Muzykantov, V.R. (2014) Nanogel carrier design for targeted drug delivery. Journal of Materials Chemistry B. 2, 8085-8097. PMCID: PMC4251498.

64) Hui, J.Z., Tsourkas, A. (2014) Optimization of photo-active Protein Z for fast and efficient site-specific conjugation of native IgG. Bioconjugate Chemistry, 25(9), 1709-1719. PMCID:PMC4166039.

63) Cheng, Z., Tsourkas, A. (2014) Monitoring phospholipase A2 activity with Gd-encapsulated phospholipid liposomes. Scientific Reports, 4, 6958. PMCID:PMC4223643.

62) Naha, P.C., Al Zaki, A., Hecht, E., Chorny, M., Chhour, P., Blankemeyer, E., Witschey, Wl, Litt, H.I., Tsourkas, A., Cormode, D.P. (2014) Dextran coated bismuth-iron oxide nanohybrid contrast agents for computed tomography and magnetic resonance imaging. Journal of Materials Chemistry B. 2, 8239-8248. PMCID: PMC4251562.

61) Chhour, P., Gallo, Cheheltani, R., Williams, D., Al Zaki, A., Paik, T, Nichol, J.L., Tian, Z., Naha, P.C., Allcock, H.R., Murray, C.B., Tsourkas, A., Cormode, D.P. (2014) Nano-disco balls: polyphosphazene nanospheres surface-loaded with diagnostically active nanocrystals. ACS Nano, 8(9), 9143-9153. PMCID:PMC4174093.

60) Karunamuni, R., Tsourkas, A., Maidment, A.D.A. (2014) Exploring silver as a contrast agent for contrast-enhanced dual-energy x-ray breast imaging. The British Journal of Radiology. 87(1041), 20140081.

59) Elias*, D.R., Crayton*, S.H., Warden, R., Tsourkas, A. (2014) Quantitative comparison of tumor delivery for multiple targeted nanoparticles simultaneously by Multiplex ICP-MS.  Scientific Reports, 4, 5840 (*contributed equally). PMCID:PMC4894420.

58) Hui, J.Z., Al Zaki, A., Cheng, Z., McNitt, C.D., Popik, V., Zhang, H., Luning Prak, E.T., Tsourkas, A. (2014) Facile method for the site-specific, covalent attachment of full-length IgG onto nanoparticles. Small, 10(16), 3354-3363. PMCID:PMC4142076.

57) Cheng, Z, Al Zaki, A., Aspinwall, C., Tsourkas, A. (2014) Stabilized porous liposomes with encapsulated Gd-labeled dextran as highly efficient MRI contrast agents. Chemical Communications, 50(19), 2502-2504. PMCID:PMC3947407.

56) Al Zaki, A., Joh, D., Cheng, Z., de Barros, A.L., Kao, G.D., Dorsey, J.F., Tsourkas, A. (2014) Gold-loaded polymeric micelles for computed tomography–guided radiation therapy treatment and radiosensitization. ACS Nano, 8(1), 104-112. PMCID: PMC3906892.

55) Song*, Y., Zhang*, X., Huang, L., Behlke, M.A., Tsourkas, A. (2014) Real-time imaging of single engineered RNA transcripts in living cells using ratiometric bimolecular beacons. Journal of Visualized Experiments, Aug 6 (90). (*contributed equally).

54) Zhang, X., Zajac, A.L., Huang, L., Behlke, M.A., Tsourkas, A. (2014) Imaging the directed transport of single engineered RNA transcripts in real-time using ratiometric bimolecular beacons. PLoS One. 9(1), e85813. PMCID:PMC3893274.

53) Nwe, K., Huang, C-H., Tsourkas, A. (2013) Gadolinium-labeled glycol chitosan as a pH-responsive magnetic resonance imaging agent for detecting acidic tumor microenvironments. Journal of Medicinal Chemistry, 56(20), 7862-7869. PMCID:PMC3880625.

52) Dorsey, J.F., Sun, L., Joh, D.Y., Witztum, A., Kao, G.D., Alonso-Basanta, M., Avery, S., Hahn, S.M., Al Zaki, A., Tsourkas, A. (2013) Gold nanoparticles in radiation research: potential applications for imaging and radiosensitization. Translational Cancer Research, 2(4), 280-291. PMCID: PMC4241969.

51) Warden-Rothman, R., Caturegli, I., Popik, V., Tsourkas, A. (2013) Sortase-Tag Expressed Protein Ligation (STEPL): combining protein purification and site-specific bioconjugation into a single step. Analytical Chemistry, 85(22), 11090-11097. PMCID: PMC3843242.

50) Joh, D.Y., Kao, G.D., Murty, S., Stangl, M., Sun, L., Al Zaki, A., Xu, X., Tsourkas, A., Dorsey, J.F. (2013) Theranostic gold nanoparticles modified for durable systemic circulation effectively and safely enhances the radiation therapy of human sarcoma cells and tumors. Translational Oncology, 6(6), 722-731. PMCID:PMC3890707.

49) de Barros, A.L., Chacko, A.M., Mikitsh, J.L, Al Zaki, A., Saboury, B., Tsourkas, A., Alavi, A. (2013) Assessment of global cardiac uptake of radiolabeled iron oxide nanoparticles in apolipoprotein E-deficient mice: implications for imaging cardiovascular inflammation. Molecular Imaging and Biology, 16(3), 330-339. PMCID:PMC4016162.

48) Zhang, X., Song, Y., Shah, A.Y., Lekova, V., Raj, A., Huang, L., Behlke, M.A., Tsourkas, A. (2013) Quantitative Assessment of Ratiometric BiMolecular Beacons as a Tool for Imaging Single Engineered RNA Transcripts and Measuring Gene Expression in Living Cells. Nucleic Acids Research, 41(15), e152. PMCID: PMC3753654.

47) Joh, D.Y., Sun, L., Stangl, M., Al Zaki, A., Murty, S., Davis, J.J., Racharla, L., Bhang, D.H., Baumann, B.C., Alonso-Basanta, M., Ryeom, S.W., Kao, G.D., Tsourkas, A., Dorsey, J.F. (2013) Selective targeting of brain tumors with nanoparticle-induced radiosensitization. PLoS One, 8(4), e62425. PMCID: PMC3640092.

46) Huang, C-H., Tsourkas, A. (2013) Gd-based macromolecules and nanoparticles as magnetic resonance contrast agents for molecular imaging. Current Topics in Medicinal Chemistry, 13(4), 411-421. PMCID: PMC3761358.

45) Elias, D. R., Poloukhtine, A., Popik, V., Tsourkas, A. (2013) Effect of ligand density, receptor density, and nanoparticle size on cell targeting. Nanomedicine. 9(2), 194-201. PMCID: PMC3502720.

44) Cheng, Z., Al Zaki, A., Hui, J.Z., Muzykantov, V.R., Tsourkas, A. (2012) Multifunctional Nanoparticles: Cost versus benefit of adding targeting and imaging capabilities. Science, 338(6109), 903-910. PMCID: PMC3660151.

43) Huang, C-H., Nwe, K., Al Zaki, A., Brechbiel, M. Tsourkas, A. (2012) Biodegradable polydisulfide dendrimer nanoclusters as MRI contrast agents. ACS Nano, 6(11), 9416-9424. PMCID: PMC3508381.

42) Cheng, Z., Al Zaki, A., Hui, J.Z., Tsourkas, A. (2012) Simultaneous quantification of tumor uptake for targeted and non-targeted liposomes and their encapsulated contents by ICP-MS. Analytical Chemistry. 84(17), 7578-7582. PMCID: PMC3443955

41) de Barros, A.L., Tsourkas, A., Saboury, B., Cardoso, V.N., Alavi, A. (2012) Emerging role of radiolabeled nanoparticles as an effective diagnostic technique. European Journal of Nuclear Medicine and Molecular Imaging, 2(1), 39. PMCID: PMC3441881.

40) Czupryna, J., Tsourkas, A. (2012) Xanthine oxidase-generated hydrogen peroxide is a consequence, not a mediator of cell death. FEBS Journal, 279(5), 844-855.

39) Crayton*, S.H., Elias*, D.R., Al-Zaki, AW, Cheng, Z., Tsourkas, A. (2012) ICP-MS analysis of lanthanide-doped nanoparticles as a non-radiative, multiplex approach to quantify biodistribution.  Biomaterials, 33(5), 1509-1519. (*contributed equally). PMCID: PMC3237748.

38) Crayton, S.H., Tsourkas, A. (2011) pH-titratable superparamagnetic iron oxide for improved nanoparticle retention in acidic tumor microenvironments. ACS Nano, 5(12), 9592-9601. PMCID: PMC3246562.

37) Cheng, Z., Elias, D.R., Kamat, N.P., Johnston, E.D., Poloukhtine, A., Popik, V., Hammer, D.A., Tsourkas, A.  (2011) Improved tumor targeting of polymer-based nanovesicles using polymer-lipid blends.  Bioconjugate Chemistry, 2(11), 2021-2029. PMCID: PMC3197750.

36) Czupryna, J., Tsourkas, A. (2011) Firefly luciferase and RLuc8 exhibit differential sensitivity to oxidative stress in apoptotic cells.  PLoS One, 6(5), e20073. PMCID: PMC3094452

35) Thorek, D.L.J, Weisshaar C.L., Czupryna, J.C., Winkelstein B.A., Tsourkas A. (2011) SPIO-enhanced detection of transient cervical nerve root compression.  Molecular Imaging, 10(3), 206-214.

34) Elias, D.R., Cheng, Z., Tsourkas, A. (2010) An Intein-Mediated Site-Specific Click Bioconjugation Strategy for Improved Tumor Targeting of Nanoparticle Systems.  Small, 6(21), 2460-2468.  PMCID: PMC3019098

33) Chen, A., Davydenko, O., Behlke, M.A., Tsourkas, A. (2010) Ratiometric BiMolecular Beacons for the sensitive detection of RNA in single living cells.  Nucleic Acids Research, 38(14), e148.  PMCID: PMC2919734

32) Thorek, D.L.J., Tsao, P.Y., Arora, V., Zhou, L., Eisenberg, R.A., Tsourkas, A. (2010) In vivo multimodal imaging of B cell distribution and response to antibody immunotherapy in mice.  PLoS One, 5(5), e10655.  PMCID: PMC2871797

31) Cheng, Z., Chen, A., Lee, H.-Y., Tsourkas, A. (2010) Examination of folate-receptor targeted liposomes with encapsulated poly(2-propylacrylic acid) as a pH-responsive nanoplatform for cytosolic drug delivery.  Small, 6(13), 1398-1401.

30) Cheng, Z., Thorek, D.L.J., Tsourkas, A. (2010) Gd-conjugated dendrimer nanoclusters as a tumor targeted T1 magnetic resonance imaging contrast agent.  Angewandte Chemie International Edition, 49(2), 346-350.  PMCID: PMC2862691

29) Warden, R., Saunders, J., Tsourkas, A. (2009) Commentary: Two-photon imaging of multicellular tumor spheroids: a novel method for evaluating the efficacy of cdc25 inhibitors.  Cancer Biology and Therapy,  8(23), 2237-2239. 

28) Chen, A.K., Behlke, M.A., Tsourkas, A. (2009) Sub-cellular trafficking and functionality of 2’-O-methyl and 2’-O-methyl-phosphorothioate molecular beacons.  Nucleic Acids Research, 37(22), e149.  PMCID: PMC2794186

27) Chen, A.K., Tsourkas, A. (2009) Imaging RNA in living cells with molecular beacons: current perspective and challenges. Journal of Innovative Optical Health Sciences, 2(4), 315-324.

26) Cheng, Z., Thorek, D.L.J, Tsourkas, A. (2009) Porous polymersomes with encapsulated Gd-labeled dendrimers as highly efficient MRI contrast agents. Advanced Functional Materials, 19(23), 3753-3759.

25) Thorek, D.L.J., Elias, D.R, Tsourkas, A. (2009) Comparative analysis of nanoparticle-antibody conjugations: carbodiimide versus click chemistry. Molecular Imaging, 8(4), 221-229. 

24) Lu, J., Tsourkas, A. (2009) Imaging individual microRNAs in single mammalian cells in situ.  Nucleic Acids Research, 37(14), e100.  PMCID: PMC2724290

23) Bao, G., Rhee, W., Tsourkas, A. (2009) Fluorescent probes for live cell RNA detection.  Annual Reviews of Biomedical Engineering, 11, 25-47.  PMCID: PMC2734976

22) Chen, A.K., Cheng, Z., Behlke, M.A., Tsourkas, A. (2008) Assessing the sensitivity of commercially available fluorophores to the intracellular environment.  Analytical Chemistry, 80(19), 7437-7444.  PMCID: PMC2626168

21) Elias, D.R., Thorek, D.L.J., Chen, A.K., Czupryna, J., Tsourkas, A. (2008) In vivo imaging of cancer biomarkers using activatable molecular probes.  Cancer Biomarkers, 4(6), 287-305. 

20) Thorek, D.L.J., Tsourkas, A. (2008) Size, charge, and concentration dependent uptake of iron oxide nanoparticles by non-phagocytic cells: a comparative study of USPIO, SSPIO, and MPIO.  Biomaterials, 29(26), 3583-3590.  PMCID: PMC2518173

19) Chen, A.K., Behlke, M.A., Tsourkas, A. (2008) Efficient cytosolic delivery of molecular beacon conjugates and flow cytometric analysis of target RNA.  Nucleic Acids Research, 36(12), e69.  PMCID: PMC2475621

18) Cheng, Z., Tsourkas, A. (2008) Paramagnetic porous polymersomes. Langmuir, 24(15), 8169-8173.  PMCID: PMC2646750

17) Zhang, C.Y., Lu, J., Tsourkas, A. (2008) Iron chelator-based amplification strategy for improved targeting of transferrin receptor with SPIO.  Cancer Biology and Therapy, 7(6), 889-895. 

16) Chen, A.K., Behlke, M.A., Tsourkas, A. (2007) Avoiding false-positive signals with nuclease-vulnerable molecular beacons in single living cells.  Nucleic Acids Research, 35(16), e105.  PMCID: PMC2018645

15) Czupryna, J., Tsourkas, A. (2006) Commentary: Suicide gene delivery by calcium phosphate nanoparticles: a novel method of targeted therapy for gastric cancer.  Cancer Biology and Therapy, 5(12), 1691-1692. 

14) Thorek, D.L.J., Chen, A.K., Czupryna, J., Tsourkas, A. (2006) Superparamagnetic iron oxide nanoparticle probes for molecular imaging.  Annals of Biomedical Engineering, 34(1), 23-38. 

13) Tsourkas*, A., Shinde-Patil*, V., Wolley, A., Allport, J., Weissleder, R. (2005) In vivo imaging of activated endothelium using anti-VCAM-1 magnetooptical probes. Bioconjugate Chemistry, 16(3), 576-581 (*contributed equally). 

12) Tsourkas, A., Newton, G., Perez, J., Basilion, J., Weissleder, R. (2005) Detection of peroxidase/H2O2-mediated oxidation with enhanced yellow fluorescent protein.  Analytical Chemistry, 77(9), 2862-2867. 

11) Kelly*, K., Allport*, J., Tsourkas, A., Shinde-Patil, V., Josephson, L., Weissleder, R. (2005) Detection of vascular adhesion molecule-1 expression using a novel multimodal nanoparticle.  Circulation Research, 96, 327-336(*contributed equally). 

10) Tsourkas, A., Weissleder, R. (2004) Illuminating the dynamics of intracellular activity with ‘active’ molecular reporters.  Mechanics and Chemistry of Biosystems, 1(2), 133-145. 

9) Tsourkas, A., Hofstetter, O., Hofstetter, H., Weissleder, R., Josephson, L. (2004) Magnetic relaxation switch immunosensors detect enantiomeric impurities.  Angewandte Chemie International Edition, 43(18), 2395-2399. 

8) Perez, J., Simeone, F., Tsourkas, A., Josephson, L., Weissleder, R. (2004) Peroxidase substrate nanosensors for MR imaging.  Nano Letters, 4(1), 119-122.

7) Bao, G., Tsourkas, A., Santangelo, P. (2004) Engineering nanostructured probes for sensitive intracellular gene detection.  Mechanics and Chemistry of Biosystems, 1(1), 23-36. 

6) Santangelo, P., Nix, B., Tsourkas, A., Bao, G. (2004) Dual FRET molecular beacons for mRNA detection in living cells.  Nucleic Acids Research, 32(6), e57.  PMCID: PMC390379

5) Tsourkas, A., Behlke, M., Xu, Y., Bao, G. (2003) Spectroscopic features of dual fluorescence/luminescence resonance energy-transfer molecular beacons.  Analytical Chemistry, 75(15), 3697-3703. 

4) Tsourkas, A., Behlke, M., Rose, S., Bao, G. (2003) Hybridization kinetics and thermodynamics of molecular beacons.  Nucleic Acids Research, 31(4), 1319-1330.  PMCID: PMC150230

3) Tsourkas, A., Bao, G. (2002) Shedding light on health and disease using molecular beacons.  Briefings in Functional Genomics and Proteomics, 1(4), 1-13. 

2) Tsourkas, A., Behlke, M., Bao, G. (2002) Structure-function relationships of shared-stem and conventional molecular beacons.  Nucleic Acids Research, 30(19), 4208-4215.  PMCID: PMC140536

1) Tsourkas, A., Behlke, M., Bao, G. (2002) Hybridization of 2’-O-methyl and 2’-deoxy molecular beacons to RNA and DNA targets.  Nucleic Acids Research, 30(23), 5168-5174.  PMCID: PMC137950


13) Wang, H.H., Tsourkas, A. (2019) Site-specific c-terminal labeling of recombinant proteins with proximity-based sortase-mediated ligation in Methods in Molecular Biology: Enzyme-mediated ligation technologies edited by Schmidt, M. and Nuijens, T., Springer, 2012, 15-28.

12) Zappala, F., Tsourkas A. (2019) Site-specific photocrosslinking to immunoglobulin G using photoreactive antibody-binding domains in Methods in Molecular Biology: Bioconjugation edited by Devoogdt, N. and Massa, S., Springer, 2033, 275-286.

11) Wang, H.H., Tsourkas, A. (2019) Overcoming the limigations of sortase with proximity-based sortase-mediated ligation (PBSL) in Methods in Molecular Biology: Proximity-labeling edited by Sunbul, M. and Jäschke, A., Springer, 2008, 165-177.

10) Yang, Y., Chen, M., Krueger, C.J., Tsourkas, A., Chen, A.K. (2018) Quantifying gene expression in living cells with ratiometric bimolecular beacons in Methods in Molecular Biology: RNA Detection, edited by Gaspar, I., Springer, pp. 231-242.

9) Crayton, S.H., Chen, A.K., Liu, J. F., Higbee-Dempsey, E.M., Huang, C.-H., Tsourkas, A., and Cheng, Z. (2017) Molecular Imaging In Comprehensive Biomaterials, edited by Ducheyne, P., Healy, K., Hutmacher, D., Kirkpatrick, J., Elsevier Inc.

8) Al Zaki, A., Cormode, D., Tsourkas, A., Dorsey, J.F. (2016) Increasing the therapeutic efficacy of radiotherapy using nanoparticles In Increasing the therapeutic ratio of radiotherapy, edited by Tofilon, P., Camphausen, K., Springer, pp. 241-265.

7) Bao, G, Wile, B., Tsourkas, A. (2013) Engineering nano-probes for live-cell imaging of gene expression In Nano and Cell Mechanics: Fundamentals and Frontiers, edited by Espinosa, H.D., Bao, G., Wiley.

6) Crayton, S.H., Chen, A.K., Cheng, Z., Tsourkas, A. (2011) Molecular Imaging In Comprehensive Biomaterials, edited by Ducheyne, P., Healy, K., Hutmacher, D., Kirkpatrick, J., Elsevier Inc.

5) Chen, A.K., Rhee, W.J., Bao, G., Tsourkas, A. (2010) Delivery of molecular beacons for live-cell imaging and analysis of RNA In Methods in Molecular Biology: Messages on the move: Techniques in RNA visualization, edited by Gerst, J.E., The Humana Press Inc.

4) Lu, J., Tsourkas, A. (2010) Quantification of miRNA abundance in single cells using locked nucleic acid-FISH and enzyme labeled fluorescence In Molecular Imaging: Methods and Protocols, edited by Shah, K., The Humana Press Inc.

3) Elias, A., Tsourkas, A. (2009) Imaging circulating cells and lymphoid tissues with iron oxide nanoparticles In Hematology American Society of Hematology Education Program, 720-726.

2) Tsourkas, A., Josephson, L. (2008) Magnetic Nanoparticles In Molecular Imaging: Principles and Practice, edited by Weissleder, R., Gambhir, S.S., Ross, B.D., Rehemtulla, A.  BC Decker Inc, Publisher.

1) Thorek, D.L.J., Czupryna, J., Chen, A.K., Tsourkas, A. (2006) Molecular imaging of cancer with superparamagnetic iron oxide nanoparticles In Cancer Imaging, edited by M.A. Hayat.  Elsevier Science/Academic Press.