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Mechanobiology
Thrombolysis
Coagulation
Bioadhesion
Gene Therapy
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Bioadhesion

We have studied receptor-mediated bioadhesion in the context of blood coagulation and inflammation reactions under conditions of hemodynamic flow. This work has focused on:

  • Platelet and neutrophil homotypic and heterotypic aggregation.
  • Platelet GPIb adhesion to vWF.
  • PSGL-1/P-selectin adhesion: Neutrophil membrane tethering.
  • Thresholds in neutrophil string formation.
  • Red blood cell adhesion to platelets, neutrophils and fibrin.
  • Pathological flow on vWF structure

 

 

 

 

 

Publications

1. 1997

Tandon P, Diamond SL. Hydrodynamic effects and receptor interactions of platelets and their aggregates in linear shear flow. Biophys J 1997; 73 (5): 2819-2835

2. 1998

Tandon P, Diamond SL. Kinetics of beta2-integrin and L-selectin bonding during neutrophil aggregation in shear flow. Biophys J 1998; 75 (6): 3163-3178

3. 2000

Diamond SL, Tandon P, Schmidtke DW, Laurenzi IJ. Cellular Aggregation in Blood Flow. Comments Theoretical Biol 2000; 8 : 1

4.

Schmidtke DW, Diamond SL. Direct observation of membrane tethers formed during neutrophil attachment to platelets or P-selectin under physiological flow. Journal of Cell Biology 2000; 149 (3): 719-729

5. 2002

Doggett TA, Girdhar G, Lawshe A, Schmidtke DW, Laurenzi IJ, Diamond SL, Diacovo TG. Selectin-like kinetics and biomechanics promote rapid platelet adhesion in flow: the GPIb(alpha)-vWF tether bond. Biophys J 2002; 83 (1): 194-205

6.

Frojmovic M, Nash G, Diamond SL. Definitions in biorheology: cell aggregation and cell adhesion in flow. Recommendation of the Scientific Subcommittee on Biorheology of the Scientific and Standardisation Committee of the International Society on Thrombosis and Haemostasis. Thromb Haemost 2002; 87 (4): 771

7.

Goel MS, Diamond SL. Adhesion of normal erythrocytes at depressed venous shear rates to activated neutrophils, activated platelets, and fibrin polymerized from plasma. Blood 2002; 100 (10): 3797-3803

8.

Park EY, Smith MJ, Stropp ES, Snapp KR, DiVietro JA, Walker WF, Schmidtke DW, Diamond SL, Lawrence MB. Comparison of PSGL-1 microbead and neutrophil rolling: microvillus elongation stabilizes P-selectin bond clusters. Biophys J 2002; 82 (4): 1835-1847

9. 2004

Kadash KE, Lawrence MB, Diamond SL. Neutrophil string formation: hydrodynamic thresholding and cellular deformation during cell collisions. Biophys J 2004; 86 (6): 4030-4039

10. 2005

Edmondson KE, Denney WS, Diamond SL. Neutrophil-bead collision assay: pharmacologically induced changes in membrane mechanics regulate the PSGL-1/P-selectin adhesion lifetime. Biophys J 2005; 89 (5): 3603-3614

11. 2008

Diamond SL, Lawrence MB, Neelamegham S. Harry L. Goldsmith, Ph.d. Ann Biomed Eng 2008; 36 (4): 523-526

12.

Ji JY, Jing H, Diamond SL. Hemodynamic regulation of inflammation at the endothelial-neutrophil interface. Ann Biomed Eng 2008; 36 (4): 586-595

13.

Oh H, Diamond SL. Ethanol enhances neutrophil membrane tether growth and slows rolling on P-selectin but reduces capture from flow and firm arrest on IL-1-treated endothelium. J Immunol 2008; 181 (4): 2472-2482

14. 2009

Flamm MH, Diamond SL, Sinno T. Lattice kinetic Monte Carlo simulations of convective-diffusive systems. J Chem Phys 2009; 130 (9): 094904

15.

Oh H, Mohler ER, 3rd, Tian A, Baumgart T, Diamond SL. Membrane cholesterol is a biomechanical regulator of neutrophil adhesion. Arterioscler Thromb Vasc Biol 2009; 29 (9): 1290-1297

16.

Schmaier AA, Zou Z, Kazlauskas A, Emert-Sedlak L, Fong KP, Neeves KB, Maloney SF, Diamond SL, Kunapuli SP, Ware J, Brass LF, Smithgall TE, Saksela K, Kahn ML. Molecular priming of Lyn by GPVI enables an immune receptor to adopt a hemostatic role. Proc Natl Acad Sci U S A 2009; 106 (50): 21167-21172

17.

Stalker TJ, Wu J, Morgans A, Traxler EA, Wang L, Chatterjee MS, Lee D, Quertermous T, Hall RA, Hammer DA, Diamond SL, Brass LF. Endothelial cell specific adhesion molecule (ESAM) localizes to platelet-platelet contacts and regulates thrombus formation in vivo. J Thromb Haemost 2009; 7 (11): 1886-1896

18.

Zhu L, Stalker TJ, Fong KP, Jiang H, Tran A, Crichton I, Lee EK, Neeves KB, Maloney SF, Kikutani H, Kumanogoh A, Pure E, Diamond SL, Brass LF. Disruption of SEMA4D ameliorates platelet hypersensitivity in dyslipidemia and confers protection against the development of atherosclerosis. Arterioscler Thromb Vasc Biol 2009; 29 (7): 1039-1045

19. 2011

Furlow M, Diamond SL. Interplay between membrane cholesterol and ethanol differentially regulates neutrophil tether mechanics and rolling dynamics. Biorheology 2011; 48 (1): 49-64

 

 

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    Institute for Medicine & Engineering (IME)
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    University of Pennsylvania
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