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Mechanobiology
Thrombolysis
Coagulation
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Coagulation

Blood coagulation is an example of time-evolving, heterotypic deposition/aggregation/fragmentation of reactive cells under flow which in turn trigger the polymerization of the fibrinogen monomer. Our laboratory is also interested in inflammation-coagulation crosstalk. These studies have investigated:

  • Neutrophil proteases as triggers of thrombosis
  • Factor VIIa function on platelets
  • High throughput phenotyping of human blood
  • Substrate mapping of coagulation proteases
  • Controlled patterned surfaces of collagen and lipidated tissue factor
  • Clot stability and fragmentation
 

 

Publications

1. 2001

Diamond SL. Reaction complexity of flowing human blood. Biophys J 2001; 80 (3): 1031-1032

2.

Goel MS, Diamond SL. Neutrophil enhancement of fibrin deposition under flow through platelet-dependent and -independent mechanisms. Arterioscler Thromb Vasc Biol 2001; 21 (12): 2093-2098

3. 2003

Goel MS, Diamond SL. Neutrophil cathepsin G promotes prothrombinase and fibrin formation under flow conditions by activating fibrinogen-adherent platelets. J Biol Chem 2003; 278 (11): 9458-9463

4. 2004

Eckmann DM, Diamond SL. Surfactants attenuate gas embolism-induced thrombin production. Anesthesiology 2004; 100 (1): 77-84

5.

Goel MS, Diamond SL. Factor VIIa-mediated tenase function on activated platelets under flow. Journal of Thrombosis and Haemostasis 2004; 2 (8): 1402-1410

6.

Goel MS, Diamond SL. Thrombosis. Encyclopedia of Biomaterials and Biomedical Engineering. 2004; (1458-1466):

7. 2005

Gosalia DN, Salisbury CM, Ellman JA, Diamond SL. High throughput substrate specificity profiling of serine and cysteine proteases using solution-phase fluorogenic peptide microarrays. Mol Cell Proteomics 2005; 4 (5): 626-636

8.

Gosalia DN, Salisbury CM, Maly DJ, Ellman JA, Diamond SL. Profiling serine protease substrate specificity with solution phase fluorogenic peptide microarrays. Proteomics 2005; 5 (5): 1292-1298

9.

Lo K, Denney WS, Diamond SL. Stochastic modeling of blood coagulation initiation. Pathophysiol Haemost Thromb 2005; 34 (2-3): 80-90

10. 2006

Gosalia DN, Denney WS, Salisbury CM, Ellman JA, Diamond SL. Functional phenotyping of human plasma using a 361-fluorogenic substrate biosensing microarray. Biotechnol Bioeng 2006; :

11.

Okorie UM, Diamond SL. Matrix protein microarrays for spatially and compositionally controlled microspot thrombosis under laminar flow. Biophys J 2006; 91 (9): 3474-3481

12. 2008

Neeves KB, Diamond SL. A membrane-based microfluidic device for controlling the flux of platelet agonists into flowing blood. Lab Chip 2008; 8 (5): 701-709

13.

Purvis JE, Chatterjee MS, Brass LF, Diamond SL. A molecular signaling model of platelet phosphoinositide and calcium regulation during homeostasis and P2Y1 activation. Blood 2008; 112 (10): 4069-4079

14. 2009

Diamond SL. Systems biology to predict blood function. J Thromb Haemost 2009; 7 Suppl 1 : 177-180

15.

Flamm MH, Diamond SL, Sinno T. Lattice kinetic Monte Carlo simulations of convective-diffusive systems. J Chem Phys 2009; 130 (9): 094904

16.

Schmaier AA, Zou Z, Kazlauskas A, Emert-Sedlak L, Fong KP, Neeves KB, Maloney SF, Diamond SL, Kunapuli SP, Ware J, Brass LF, Smithgall TE, Saksela K, Kahn ML. Molecular priming of Lyn by GPVI enables an immune receptor to adopt a hemostatic role. Proc Natl Acad Sci U S A 2009; 106 (50): 21167-21172

17.

Stalker TJ, Wu J, Morgans A, Traxler EA, Wang L, Chatterjee MS, Lee D, Quertermous T, Hall RA, Hammer DA, Diamond SL, Brass LF. Endothelial cell specific adhesion molecule (ESAM) localizes to platelet-platelet contacts and regulates thrombus formation in vivo. J Thromb Haemost 2009; 7 (11): 1886-1896

18.

Zhu L, Stalker TJ, Fong KP, Jiang H, Tran A, Crichton I, Lee EK, Neeves KB, Maloney SF, Kikutani H, Kumanogoh A, Pure E, Diamond SL, Brass LF. Disruption of SEMA4D ameliorates platelet hypersensitivity in dyslipidemia and confers protection against the development of atherosclerosis. Arterioscler Thromb Vasc Biol 2009; 29 (7): 1039-1045

19. 2010

Chatterjee MS, Denney WS, Jing HY, Diamond SL. Systems Biology of Coagulation Initiation: Kinetics of Thrombin Generation in Resting and Activated Human Blood. Plos Computational Biology 2010; 6 : 9

20.

Chatterjee MS, Purvis JE, Brass LF, Diamond SL. Pairwise agonist scanning predicts cellular signaling responses to combinatorial stimuli. Nat Biotechnol 2010; 28 (7): 727-732

21.

Diamond SL. Tissue factor activity under flow. Thromb Res 2010; 125 Suppl 1 : S29-30

22.

Maloney SF, Brass LF, Diamond SL. P2Y12 or P2Y1 inhibitors reduce platelet deposition in a microfluidic model of thrombosis while apyrase lacks efficacy under flow conditions. Integr Biol (Camb) 2010; 2 (4): 183-192

23.

Neeves KB, Illing DA, Diamond SL. Thrombin flux and wall shear rate regulate fibrin fiber deposition state during polymerization under flow. Biophys J 2010; 98 (7): 1344-1352

24.

Signarvic RS, Cierniewska A, Stalker TJ, Fong KP, Chatterjee MS, Hess PR, Ma P, Diamond SL, Neubig RR, Brass LF. RGS/Gi2alpha interactions modulate platelet accumulation and thrombus formation at sites of vascular injury. Blood 2010; 116 (26): 6092-6100

25.

Wannemacher KM, Zhu L, Jiang H, Fong KP, Stalker TJ, Lee D, Tran AN, Neeves KB, Maloney S, Kumanogoh A, Kikutani H, Hammer DA, Diamond SL, Brass LF. Diminished contact-dependent reinforcement of Syk activation underlies impaired thrombus growth in mice lacking Semaphorin 4D. Blood 2010; 116 (25): 5707-5715

26. 2011

Colace T, Falls E, Zheng XL, Diamond SL. Analysis of morphology of platelet aggregates formed on collagen under laminar blood flow. Ann Biomed Eng 2011; 39 (2): 922-929

27.

Colace TV, Jobson J, Diamond SL. Relipidated tissue factor linked to collagen surfaces potentiates platelet adhesion and fibrin formation in a microfluidic model of vessel injury. Bioconjug Chem 2011; 22 (10): 2104-2109

28.

Stolla M, Stefanini L, Roden RC, Chavez M, Hirsch J, Greene T, Ouellette TD, Maloney SF, Diamond SL, Poncz M, Woulfe DS, Bergmeier W. The kinetics of alphaIIbbeta3 activation determines the size and stability of thrombi in mice: implications for antiplatelet therapy. Blood 2011; 117 (3): 1005-1013

29. 2012

Colace TV, Muthard RW, Diamond SL. Thrombus growth and embolism on tissue factor-bearing collagen surfaces under flow: role of thrombin with and without fibrin. Arterioscler Thromb Vasc Biol 2012; 32 (6): 1466-1476

30.

Flamm MH, Diamond SL. Multiscale systems biology and physics of thrombosis under flow. Ann Biomed Eng 2012; 40 (11): 2355-2364

31.

Welsh JD, Colace TV, Muthard RW, Stalker TJ, Brass LF, Diamond SL. Platelet-targeting sensor reveals thrombin gradients within blood clots forming in microfluidic assays and in mouse. J Thromb Haemost 2012; :

32. 2013

Colace TV, Diamond SL. Direct observation of von Willebrand factor elongation and fiber formation on collagen during acute whole blood exposure to pathological flow. Arterioscler Thromb Vasc Biol 2013; 33 (1): 105-113

33.

Jaeger DT, Diamond SL. Pairwise agonist scanning-flow cytometry (PAS-FC) measures inside-out signaling and patient-specific response to combinatorial platelet agonists. Biotechniques 2013; 54 (5): 271-277

34.

Stalker TJ, Traxler EA, Wu J, Wannemacher KM, Cermignano SL, Voronov R, Diamond SL, Brass LF. Hierarchical organization in the hemostatic response and its relationship to the platelet-signaling network. Blood 2013; 121 (10): 1875-1885

35.

Voronov RS, Stalker TJ, Brass LF, Diamond SL. Simulation of intrathrombus fluid and solute transport using in vivo clot structures with single platelet resolution. Ann Biomed Eng 2013; 41 (6): 1297-1307

 

 

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